Histone Variants

Methods for Preparing Nucleosomes Containing Histone Variants.- Characterization of Post-Translational Modifications on Histone Variants.- Purification of Histone Variant-Interacting Chaperone Complexes.- Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics.- Histone Native Chromatin Immunprecipitation.- How to Tackle Challenging ChIP-seq, with Long-Range Cross-Linking, using ATRX as an Example.- Time-ChIP : A Method to Determine Long-Term Locus-Specific Nucleosome Inheritance.- MINCE-seq : Mapping In Vivo Nascent Chromatin with EdU and Sequencing.- RChIP-seq : Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes.- Bioinformatics Analysis of Nucleosome and Histone Variant Positioning.- Imaging newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Tag System.- Real Time de novo Deposition of Centromeric Histone-Associated Proteins Usingthe Auxin Inducible Degradation System.- Live Imaging of Parental Histone Variant Dynamics in UVC-Damaged Chromatin.- CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A.- Studying the Evolution of Histone Variants Using Phylogeny.- Characterization of Post-Meiotic Male Germ Cell Genome Organizational States.- An Animal Model for Genetic Analysis of Multi-Gene Families : Cloning and Transgenesis of Large Tandemly-Repeated Histone Gene Clusters.- Imaging and Quantitation of Assembly Dynamics of the Centromeric Histone H3 Variant CENP-A in Drosophila melanogaster Spermatocytes by Immunofluorescence and Fluorescence In-Situ Hybridization (Immuno-FISH).