Closer Look at Calorimetry

In this compilation, the authors review the planning of multithermal titration calorimetry experiments using triosephosphate isomerase as a case study with two of its inhibitors, 2PG and PGH, under physiological conditions and osmotic stress. Following this, the authors revisit classic and disequilibrium calorimetry by presenting and discussing raw data obtained with a thermocouple probe and a beaker filled with water and placed in an ultrasonic cleaning bath. Both calorimetric methods are compared and evaluated. A combination of temperature-dependent UV spectroscopy, differential scanning calorimetry and circular dichroism techniques are explored for the investigation of their thermodynamic contribution to the incorporation of bulges and mismatches in duplex DNA. The authors also attempt to expound how to apply electrochemical Peltier heat to investigate the thermodynamics of electrochemical reactions and determine their thermodynamic parameters. A strict definition of electrochemical Peltier is given, and its generation and characteristics as a reversible heat are discussed. The closing chapter summarizes existing energy expenditure measurement methods, discussing their influencing factors and applicability in different populations. Considering the importance of accurate expenditure in terms of health promoting strategies, this collection provides important insight into the determinants of appropriate energy expenditure estimation.