In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs

Preface.- Part. Overview.- Chapter 1. Introduction.- Chapter 2. Biomedical significance and development of "IVCT".- Chapter 3. How to perform 'IVCT'.- Chapter 4. Technical merits with 'IVCT'.- Part ?. Application of 'IVCT' to various mouse organs.- Chapter 5. Histochemical Analyses of Living Mouse Liver under Different Hemodynamic Conditions.- Chapter 6. Three-dimensional reconstruction of liver tissues with confocal laser scanning microscopy.- Chapter 7. Application of "in vivo cryotechnique" to immunohistochemical detection of hypoxia in mouse liver tissues treated with pimonidazole.- Chapter 8. Immunohistochemical detection of soluble immunoglobulins in small intestines.- Chapter 9. Detection of MAPK signal transduction proteins in an ischemia/reperfusion model of small intestines.- Chapter 10. Histological Study and LYVE-1 Immunolocalization of Mesenteric Lymph Nodes.- Chapter 11. Distribution of immunoglobulin-producing cells in immunized mouse spleens.- Chapter12. Alteration of erythrocyte shapes in various organs of living mice or under blood flow conditions.- Chapter 13. Dynamic ultrastructure of smooth muscle cells in dystrophin-deficient mdx or normal scn mice.- Chapter 14. Dynamic Bioimaging of Serum Proteins in Beating Mouse Hearts.- Chapter 15. Histochemical analyses and quantum dot imaging of microvascular blood flow with pulmonary edema.- Chapter 16. Dynamic ultrastructure of pulmonary alveoli of living mice under respiratory conditions.- Chapter 17. Immunohistochemical analysis of various serum proteins in living mouse thymus.- Chapter 18. Immuno- and enzyme-histochemistry of HRP for demonstration of blood vessel permeability in mouse thymic tissues.- Chapter 19. Immunolocalization of serum proteins in living mouse glomeruli under various hemodynamic conditions.- Chapter 20. Immunohistochemical Analyses on Albumin and IgG in Acute Hypertensive Mouse Kidneys.- Chapter 21. Application of novel "in vivo cryotechnique" in living animal kidneys.-Chapter 22. Application of periodic acid-Schiff fluorescence emission for imunohistochemistry of living mouse renal glomeruli.- Chapter 23. Immunohistochemical analyses on serum proteins in nephrons of protein-overload mice.- Chapter 24. Immunohistochemical analyses of serum proteins in puromycin aminonucleoside nephropathy of living rat kidneys.- Chapter 25. Immunolocalization of phospho-Arg-directed protein kinase-substrate in hypoxic kidneys.- Chapter 26. Dynamic ultrastructures of renal glomeruli in living mice under various hemodynamic conditions.- Chapter 27. Function of dynamin-2 in formation of discoid vesicles in urinary bladder umbrella cells.- Chapter 28. Involvement of follicular basement membrane and vascular endothelium in blood-follicle barrier formation of mice.- Chapter 29. Permselectivity of blood-follicle barriers in mouse polycystic ovary model.- Chapter 30. Application of "in vivo cryotechnique" to immunohistochemical study of serum albumin in normal and cadmium-treated mouse testis organs.- Chapter 31. Immunohistochemical detection of angiotensin II receptors in mouse cerebellum.- Chapter 32. Application of "in vivo cryotechnique" to immunohistochemical analyses for effects of anoxia on serum immunoglobulin and albumin leakage through blood-brain barrier in mouse cerebellum.- Chapter 33. Extracellular Space in Central Nervous System.- Chapter 34. Overview on recent applications of in vivo cryotechnique in neurosciences.- Chapter 35. Application of "in vivo cryotechnique" to immunohistochemical detection of phosphorylated rhodopsin in light-exposed retina of living mouse.- Chapter 36. Application of "in vivo cryotechnique" to immunohistochemical detection of glutamate in mouse retina inner segment of photoreceptors.- Chapter 37. Application of "in vivo cryotechnique" to immunohistochemical study of mouse sciatic nerves under various stretching conditions.- Part ?. Further preparation after 'IVCT'.- Chapter 38. Freeze-substitution (FS) fixation method.- Part IV. Comments on advantages of 'IVCT'.- Chapter 39. Cryotechniques and freeze-substitution for immunostaining of intranuclear antigens and fluorescence in situ hybridization.- Chapter 40. Replica immunoelectron microscopy for caveolin in living smooth muscle cells.- Chapter 41. Application of "in vivo cryotechnique" to detection of injected fluorescence-conjugated IgG in living mouse organs.- Chapter 42. Application of "in vivo cryotechnique" to visualization of microvascular blood ?ow in mouse kidney by quantum dot injection.- Part V . Direct detection of molecules by Raman microscopy.- Chapter 43. Application of "in vivo cryotechnique" to Raman microscopy of freeze-dried mouse eyeball-slice.- Chapter 44. Application of ''in vivo cryotechnique" to detection of erythrocyte oxygen saturation in mouse blood vessels with confocal Raman cryomicroscopy.- Part VI . Future development for human pathology.- Chapter 45. Imaging of thrombosis and microcirculationin mouse lungs of initial melanoma metastasis.- Chapter 46. Differential distribution of blood-derived proteins in xenografted human adenocarcinoma tissues.- Part VII . New cryobiopsy invention.- Chapter 47. Morphological and Histochemical Analysis of Living Mouse Livers by New 'Cryobiopsy' Technique.- Chapter 48. Recent Development of In Vivo Cryotechnique to Cryobiopsy for Living Animals.- Part VIII . Application of cryobiopsy for living animal tissues.- Chapter 49. Significance of cryobiopsy for morphological and immunohistochemical analyses of xenografted human lung cancer tissues and functional blood vessels.- Chapter 50. Immunolocalization of serum proteins in xenografted mouse model of human tumor cells.- Chapter 51. Histochemical approach of cryobiopsy for glycogen distribution in living mouse livers under fasting and local circulation loss conditions.- Part IX . New bioimaging of living animal tissues.- Chapter 52. Bioimaging of fluorescence-labeled mitochondria in subcutaneously grafted murine melanoma cells by the "in vivo cryotechnique" .- Chapter 53. Application of "in vivo cryotechnique" to visualization of ATP with luciferin-luciferase reaction in mouse skeletal muscles.- Chapter 54. Concluding remarks.