Amphibians

Name: Amphibians | Guidelines for the Breeding, Care and Management of Laboratory Animals
Brand: National Academies Press
Price: 86.99 EUR
Availability: OnlineOnly

Guidelines for the Breeding, Care and Management of Laboratory Animals

National Research Council Institute for Laboratory Animal Research Committee on Standards Subcomm(Autor*in)

National Academies Press

1. Auflage

Erschienen am 1. Januar 1974

162 Seiten

E-Book

PDF mit Adobe-DRM

Systemvoraussetzungen

978-0-309-54015-5 (ISBN)

86,99 €inkl. 7% MwSt.

Systemvoraussetzungen

für PDF mit Adobe-DRM

E-Book Einzellizenz

Als Download verfügbar

Weitere Details

Weitere Ausgaben

Inhalt

Amphibians
Copyright
Preface
Contents
I Introduction
A. GENERAL COMMENTS
B. THE NEED
1. The Demand
2. The Supply
a. The Natural Resource
b. Artificial Culture
3. Recommendations
C. USERS OF AMPHIBIANS
D. LABORATORY-DEFINED AMPHIBIANS
II Classification and Description of Amphibians Commonly Used for Laboratory Research
A. PHYLOGENY AND CLASSIFICATION
B. DESCRIPTION OF SPECIES COMMONLY USED IN LABORATORY RESEARCH
1. Urodeles
a. Ambystoma (2N = 28
ZW - ZZ , but two triploid species are known)
b. Notophthalmus viridescens (2N = 22
ZW - ZZ)
c. Necturus maculosus (2N = 24
ZW - ZZ)
2. Anurans
a. Xenopus laevis (2N = 36
ZW - ZZ)
b. Rana catesbeiana (2N = 26
XX - XY)
c. Rana grylio (2N = 26
XX - XY)
d. Rana clamitans (2N = 26
XX - XY)
e. Rana pipiens (2N = 26
XX - XY)
f. Rana palustris (2N = 26
XX - XY)
g. Rana sylvatica (2N = 26
XX - XY)
h. Bufo marinus (2N = 22
XX - XY)
i. Bombina orientalis (2N = 24
XX - XY)
III Definition and Description of Experimental Amphibians
A. INTRODUCTION
B. DEFINITION
1. Wild
2. Wild Caught
a. Nonconditioned
b. Conditioned
3. Laboratory-Reared
a. Standard
b. Miscellaneous
4. Laboratory-Bred
a. Standard
b. Miscellaneous
C. DESCRIPTION OF LABORATORY-REARED AND LABORATORY-BRED AMPHIBIANS
1. Types of Populations and Lines
a. Random Mating Lines
b. Heterozygous Isogenic Clones
c. Heterozygous Marked Lines
d. Mutant Lines
e. Inbred Lines
f. Gynogenetic Diploid Lines
g. Homozygous Lines
h. Haploid Animals
i. Polyploid Animals
2. Sex Determination and Its Manipulation
3. Species of Laboratory-Reared or Laboratory-Bred Amphibians Available in the United States
a. Anurans
b. Urodeles
IV Sources
A. SELECTION
B. DEALER CARE
C. ORDERING, SHIPPING, AND RECEIVING
1. Ordering
2. Shipping
3. Receiving
a. Aquatic Amphibians
b. Terrestrial Amphibians
D. LEGAL ASPECTS
V Physical Facilities
A. RELATION TO NATURAL HABITAT
B. THE AMPHIBIAN QUARTERS
1. General Description
a. Isolation Quarters
b. Heating, Ventilation, and Size Specifications for Rooms
c. Description of Ancillary Rooms
d. General Specifications
2. Environmental Control
a. Water
b. Temperature
c. Lighting
C. ENCLOSURES
1. Embryos: Fertilization through Initiation of Feeding
2. Larvae
3. Juveniles
a. At Metamorphic Climax
b. Postmetamorphic
4. Adult Enclosures (Evaluation Criteria)
5. Hibernation Quarters
6. Enclosure Designs
a. The Amphibian Facility of The University of Michigan
b. The R. catesbeiana Facility at Louisiana State University
c. Southern Frog Company (J. M. Priddy, Dumas, Arkansas)
d. The Aquatic Animal Facility of Arizona State University
VI Amphibian Management and Laboratory Care
A. GENERAL COMMENTS
B. ANURANS
1. Ranidae
a. R. pipiens
b. R. caresbeiana
c. Other Ranid Species
2 Other Anurans
a. Xenopus
b. Bufo
c. Bombina orientalis
C. URODELA
1. Axolotls (Neotenic Larvae of Ambystoma mexicanum)
a. Early Larvae
b. Mature Larvae
2. Other Urodeles
VII Breeding
A. ANURANS
1. General Comments
a. Sexing
b. Readiness for Reproduction
c. Identification of Individuals
2. Artificial Induction of Ovulation
3. Amplexus
4. Artificial Insemination
5. Parthenogenetic Lines and Haploid Animals
6. Nomozygous Lines and Androgenesis
7. Polyploid Animals
8. Mosaic Animals
9. Nuclear Transplantation
10. Xenopus
Procurement of Gametes
Artificial Insemination
B. URODELES
1. Mating
2. Spawning
3. Artificial Insemination
4. Initial Care of Embryos
VIII Records and Information Control
A. IDENTIFICATION ON INDIVIDUALS
1. Numbering
2. Tattooing
3. Branding
4. Toe Clipping
5. Other Marking Systems
6. Drawings and Photographs
B. INFORMATION CONTROL SYSTEMS
IX Amphibian Medicine
A. GENERAL COMMENTS
B. BACTERIAL DISEASES
1. Pathogens
2. Drug Selection and Administration
3. Identifying Diseased Frogs
4. The Need for Treatment
C. VIRAL DISEASES
1. General Comments
2. Lucké Tumor Herpesvirus (LTHV)
3. Amphibian Polyhedral Cytoplasmic Deoxyribovirus (PCDV)
4. Lymphosarcoma Virus of Xenopus
D. PARASITIC DISEASES
E. MYCOTIC DISEASES
F. EUTHANASIA AND ANESTHESIA
X Personnel
A. GENERAL COMMENTS
B. SAFETY HAZARDS
1. Physical
2. Biological
Appendix A Status of "Endangered" Amphibians
Appendix B Control Laws by States and Canadian Provinces
UNITED STATES
CANADIAN PROVINCES
References

Systemvoraussetzungen

Als PDF speichern Als Link merken