Integrative Structural Biology with Hybrid Methods

Part-1.Introduction and historical background.- Chapter 1.Overall introduction and rationale, with View from computational biology.- Chapter 2.Integrative/Hybrid Methods Structural Biology: Role of Macromolecular Crystallography.- Chapter 3.View from nuclear magnetic resonance spectroscopy.- Part- 2.New experimental tools enabling hybrid methods.- Chapter 4.Complementary use of electron cryomicroscopy and X-ray crystallography: Structural studies of actin and actomyosin filaments.- Chapter 5.Current solution NMR techniques for structure-function studies of proteins and RNA molecules.- Chapter 6.The PA tag: a versatile peptide tagging system in the era of integrative structural biology.- Chapter 7.Small angle scattering and structural biology: Data quality and model validation.- Chapter 8.Structural investigation of proteins and protein complexes by chemical cross-linking/mass spectrometry.- Chapter 9.Prediction of structures and interactions from genome information.- Chapter10.A hybrid approach for protein structure determination combining sparse NMR with evolutionary coupling sequence data (EC-NMR).- Chapter 11.Harnessing the combined power of SAXS and NMR.- Chapter 12.2D Hybrid analysis.- Part-3.New computational tools enabling hybrid methods.- Chapter 13.Hybrid methods for macromolecular modeling by molecular mechanics simulations with experimental data.- Chapter 14.Rigid-body fitting of atomic models on 3D density maps of electron microscopy.- Chapter 15.Hybrid methods for modeling protein structures using molecular dynamics simulations and small-angle X-ray scattering data.- Part-4.Data validation and archives for hybrid methods.- Chapter 16.Archiving pf integrative/hybrid models.