This detailed volume encompasses chapters from leading
experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional
production and stabilization of recombinant integral membrane proteins (IMPs). Membrane
proteins play a key role in numerous pathologies such as cancer, cystic
fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on
these and other disorders are hampered by a lack of information about the
proteins involved. This book sets out to aid researchers in rectifying this
situation. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions
to their respective topics, lists of the necessary materials and reagents,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative and up-to-date,
Heterologous
Expression of Membrane Proteins: Methods and Protocols, Second Edition
serves as an ideal guide for scientists attempting to delve deeper into the myriad
unique IMP structures.
Reihe
Auflage
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
39
27 s/w Abbildungen, 39 farbige Abbildungen
XII, 322 p. 66 illus., 39 illus. in color.
ISBN-13
978-1-4939-3637-3 (9781493936373)
DOI
10.1007/978-1-4939-3637-3
Schweitzer Klassifikation
Cell-Free Production of Membrane Proteins in
Escherichia coli
Lysates for Functional
and Structural Studies.- Membrane Protein Production in the Yeast,
S. cerevisiae
.- Membrane Proteins
Production in
Escherichia coli
:
Protocols and Rules.- Codon Optimizing for Increased Membrane Protein
Production: A Minimalist Approach.- Generation of Tetracycline-Inducible
Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane
Proteins.- Membrane Protein Production in
Lactococcus lactis
for Functional
Studies.- Expression of
Viral Envelope Glycoproteins in
Drosophila melanogaster
S2 Cells.-
Leishmania tarentolæ
as a Promising Tool
for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane
Proteins: The Case of the ABC Pump ABCG6.- Overexpression, Membrane
Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA.- Expression of
Eukaryotic Membrane Proteins in
Pichia
pastoris
.- Integral Membrane Protein Expression in
Saccharomyces cerevisiae
.- High-Throughput Baculovirus Expression
System for Membrane Protein Production.- Small-Scale Screening to Large-Scale
Over-Expression of Human Membrane Proteins for Structural Studies.- Purification
of Human and Mammalian Membrane Proteins Expressed in
Xenopus laevis
Frog Oocytes for Structural Studies.- Membrane
Protein Solubilization and Composition of Protein Detergent Complexes.- Detergent-Free
Membrane Protein Purification.- Conformational Dynamics and Interactions of
Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry.- Lessons from an a-Helical Membrane Enzyme: Expression,
Purification, and Detergent Optimization for Biophysical and Structural
Characterization.- Method to Screen Multidrug Transport Inhibitors Using Yeast
Over-Expressing a Human MDR Transporter.
