Target Identification and Validation in Drug Discovery

Using Functional Genetics in Haploid Cells for Drug Target Identification.- Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug-Target Interactions.- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development.- CellProfiler and KNIME: Open Source Tools for High Content Screening.- In Silico Target Druggability Assessment: From Structural to Systemic Approaches.- In Silico Drug-Target Profiling.- Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies.- Expression of Human VH Single Domains as Fc-Fusions in Mammalian Cells.- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs).- 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration.- Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery.- In Vivo Pharmacology Models for Cancer Target Research.- Use of CRISPR/Cas9 for the Modification of the Mouse Genome.- Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction.- Creation of PDX-Bearing Humanized Mice to Study Immuno-Oncology.- Immunophenotyping of Tissue Samples Using Multicolour Flow Cytometry.- Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers.- Tangential Flow Filtration with or without Subsequent Bind Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles.- Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or Rearrangements) Combined with Protein Overexpression in Clinical Samples.