Cellular and Molecular Methods in Neuroscience Research

Analyses of Intracellular Signal Transduction Pathways in CNS Progenitor Cells.- Confocal and Electron Microscopic Tracking of Internalized Neuropeptides and/or Their Receptors.- Transfection Methods for Neurons in Primary Culture.- Polyethylenimine: A Versatile Cationic Polymer for Plasmid-Based Gene Delivery in the CNS.- Transfection of GABAA Receptor with GFP-Tagged Subunits in Neurons and HEK 293 Cells.- Neuronal Transfection Using Particle-Mediated Gene Transfer.- Analysis of Gene Expression in Genetically Labeled Single Cells.- Immunocytochemistry and In Situ Hybridization: Their Combinations for Cytofunctional Approaches of Central and Peripheral Neurons.- In Situ Reverse Transcription PCR for Detection of mRNA in the CNS.- Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronal Connectivity.- Combined Electrophysiological and Morphological Analyses of CNS Neurons.- Tract Tracing Methods at the Light Microscopic Level.- Tract Tracing Methods at the Ultrastructural Level.- In Vivo Analysis of Cell Proliferation and Apoptosis in the CNS.- Confocal Imaging of Nerve Cells and Their Connections.- Confocal Imaging of Calcium Signaling in Cells from Acute Brain Slices.