Characterization of Deubiquitinase Catalytic State Using a Structure-Based Approach.- Synthetic Diubiquitin Fluorogenic Substrates to Study DUBs.- Fluorometric Characterization of DUB Activity: From Single Enzyme Reactions to Live Intact Cell.- Using Activity-Based Proteomics for the Quantification of Deubiquitinases in Animal Tissue.- Design and Synthesis of Ubiquitin-Based Chemical tools with Unnatural Amino Acids for Selective Detection of Deubiquitinases.- High Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity.- Activity-Based Protein Profiling (ABPP) for Cellular Deubiquitinase (DUB) and Inhibitor Profiling at Deep and High-Throughput Levels.- High-Throughput MALDI-TOF Mass-Spectrometry-based Deubiquitylating Enzyme Assay for Drug Discovery.- Bioinformatical Approaches to The Discovery and Classification of Novel Deubiquitinases.- Application of Archaea in Deubiquitinase-Like Enzyme Discovery and Activity Assay.- Characterization of the Ubiquitin and ISG15 Deconjugase Activity of SARS-CoV-1 and 2 Papain-like Protease.- Next-generation Phage Display to Identify Peptide Ligands of Deubiquitinases.- Identification of Deubiquitinase Substrates in
Xenopus
Egg Extract.- Identification of Deubiquitinase Substrates in
Saccharomyces cerevisiae
by Systematic Overexpression.- Real-time Deubiquitination Assays using a Free-Ubiquitin Sensor.- A Method to Monitor Activity of SARS-CoV-2 Nsp3 from Cells.- Use of Deubiquitinase Fusion Proteins to Characterize Endocytic Trafficking in Yeast.
