Aptamers in Bioanalysis

Name: Aptamers in Bioanalysis
Brand: Wiley
Price: 146.99 EUR
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M. Mascini(Autor*in)

Wiley (Verlag)

Erschienen am 28. April 2009

336 Seiten

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Inhalt

Intro
APTAMERS IN BIOANALYSIS
CONTENTS
PREFACE
CONTRIBUTORS
I INTRODUCTION
1 APTAMERS: LIGANDS FOR ALL REASONS
1.1 Introduction
1.2 The Power of Selection and Aptamer Refinement
1.3 The Chemistry Drives the Shape
1.4 Aptaregulators
1.5 Aptasensors
1.6 Prospects
References
2 SELEX AND ITS RECENT OPTIMIZATIONS
2.1 Introduction
2.2 Aptamers and Their Selection by SELEX
2.3 Modifications of SELEX Technology
2.4 Advantages and Limitations of Aptamers and Their Selection Technology
2.5 Applications of Aptamers Being Developed for the Market
2.6 Future Perspectives
References
II BIOSENSORS
3 ELECTROCHEMICAL APTASENSORS
3.1 Introduction
3.2 Electrochemical Aptasensor Based on Redox-Active Aptamer Monolayers Linked to Electrodes
3.3 Enzyme-Based Amplified Electrochemical Aptasensors
3.4 Amplified Electrochemical Aptasensors Based on Nanoparticles
3.5 Label-Free Electrochemical Aptasensors
3.6 Field-Effect Transistor-Based Aptasensors
3.7 Conclusions and Perspectives
References
4 APTAMERS: HYBRIDS BETWEEN NATURE AND TECHNOLOGY
4.1 Introduction
4.2 Specific Features of Aptamers
4.3 Electrochemical Detection of Nucleic Acids
4.4 Cytochrome c Binding by Aptamers
4.5 DNA Machines and Aptamers
References
5 DETECTION OF PROTEIN-APTAMER INTERACTIONS BY MEANS OF ELECTROCHEMICAL INDICATORS AND TRANSVERSE SHEAR MODE METHOD
5.1 Introduction
5.2 Immobilization of Aptamers on a Solid Support
5.3 Detection of Aptamer-Ligand Interactions
5.3.1 Electrochemical Methods
5.3.2 Acoustic Methods
5.4 Conclusions
References
6 BIOSENSORS USING THE APTAMERIC ENZYME SUBUNIT: THE USE OF APTAMERS IN THE ALLOSTERIC CONTROL OF ENZYMES
6.1 Aptamers as Molecular Recognition Elements of Biosensors
6.1.1 Comparing Aptamers to Antibodies
6.1.2 Signaling Aptamers
6.2 Homogeneous Sensing
6.2.1 Biosensor Systems That Do Not Require Bound-Free Separation
6.2.2 Aptameric Enzyme Subunit
6.3 Evolution-mimicking Algorithm for the Improvement of Aptamers
References
7 NANOMATERIAL-BASED LABEL-FREE APTASENSORS
7.1 Introduction
7.2 Label-Free Electrochemical Aptasensors
7.3 Field-Effect Transistor-Based Aptasensors
7.4 Label-Free Aptasensors Based on Localized Surface Plasmon Resonance
7.5 Forthcoming Challenges and Concluding Remarks
References
8 APTAMER-BASED BIOANALYTICAL ASSAYS: AMPLIFICATION STRATEGIES
8.1 Introduction
8.2 Bioanalytical Assays Based on Aptamer-Functionalized Nanoparticles
8.3 Aptamers and Quantum Dot-Based Assays
8.4 Aptazymes and Aptamer-Based Machines
8.5 Polymerase Chain Reaction as an Amplification Method in Aptamer-Based Assays
8.6 Conclusions
References
III APPLICATIONS
9 KINETIC CAPILLARY ELECTROPHORESIS FOR SELECTION, CHARACTERIZATION, AND ANALYTICAL UTILIZATION OF APTAMERS
9.1 Introduction
9.1.1 Kinetic Capillary Electrophoresis
9.1.2 The Concept of NECEEM and ECEEM
9.2 Selection of Aptamers Using KCE Methods for Partitioning and Affinity Control
9.2.1 NECEEM-Based Selection of Aptamers
9.2.2 ECEEM-Based Selection of Aptamers
9.2.3 Optimization of PCR
9.2.4 Future of KCE Methods for Aptamer Selection
9.3 Measurements of Binding Parameters of Target-Aptamer Interaction by KCE Methods
9.3.1 Foundation
9.3.2 Temperature Control Inside the Capillary
9.3.3 Examples
9.4 Quantitative Affinity Analysis of a Target Using Aptamer as an Affinity Probe
9.4.1 Foundation
9.4.2 Example
9.5 Conclusions
References
10 APTAMERS FOR SEPARATION OF ENANTIOMERS
10.1 Introduction
10.2 Generation and Properties of Enantioselective Aptamers
10.3 Immobilized Aptamers for Enantiomeric Separation by Liquid Chromatography
10.3.1 Stationary-Phase Preparation and Column Packing
10.3.2 DNA Aptamer-Based CSPs
10.3.3 RNA Aptamer-Based CSPs and the Mirror-Image Strategy
10.3.4 Class-Specific Aptamer-Based CSPs
10.4 Aptamers for Analysis of Enantiomers by Capillary Electrophoresis
10.4.1 Aptamers as Chiral Additives in the Background Electrolyte for CE Enantiomeric Separation
10.4.2 Aptamers for the Design of an Affinity CE-Based Enantioselective Competitive Assay
10.5 Conclusions
References
11 APTAMER-MODIFIED SURFACES FOR AFFINITY CAPTURE AND DETECTION OF PROTEINS IN CAPILLARY ELECTROPHORESIS AND MALDI-MASS SPECTROMETRY
11.1 Introduction
11.2 Aptamer-Modified Capillaries in Affinity Capillary Electrophoresis
11.3 Aptamer-Modified Surfaces for Affinity MALDI-MS
11.3.1 Overview
11.3.2 Affinity MALDI-MS of Thrombin
11.3.3 Affinity MALDI-MS of IgE
11.3.4 Summary
11.4 Beyond Aptamers: Genome-Inspired DNA Binding Ligands
References
12 STRATEGY FOR USE OF SMART ROUTES TO PREPARE LABEL-FREE APTASENSORS FOR BIOASSAY USING DIFFERENT TECHNIQUES
12.1 Introduction
12.2 Electrochemical Aptasensors
12.2.1 POSOALF Mode
12.2.2 PFSOALF Mode
12.2.3 Electrochemical Impedimetric Aptasensors
12.2.4 Electrochemical Aptasensors with Nonlabeled Redox Probes
12.3 Fluorescent Molecular Switches
12.3.1 POSFALF Mode
12.3.2 PFSFALF Mode
12.4 Colorimetry
12.4.1 POSFALF Mode
12.4.2 PFSFALF Mode
12.5 Hemin-Aptamer DNAzyme-Based Aptasensor
12.6 Liquid Chromatography, Electrochromatography, and Capillary Electrophoresis Applications
12.7 Other Aptasensors
12.8 Conclusions
References
INDEX

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