Chromatin Structure and Histones1. In Vitro Chromatin Assembly - Strategies and Quality ControlUma Muthurajan, Francesca Mattiroli, Serge Bergeron, Keda Zhou, Yajie Gu, Srinivas Chakravarthy, Pamela Dyer, Thomas Irving and Karolin Luger2. Preparation, Crystallization and Structure Determination of Chromatin Enzyme/Nucleosome ComplexesRobert K. McGinty, Ravindra D. Makde and Song Tan3. Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Non-Histone Centromere ProteinsNikolina Sekulic and Ben E. Black4. Functional Characterization of Histone Chaperones using SNAP-Tag-Based Imaging to Assess De Novo Histone DepositionCamille Clément, Isabelle Vassias, Dominique Ray-Gallet and Geneviève Almouzni5. Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodelling EnzymesCoral Y. Zhou and Geeta J. Narlikar
Posttranslational Histone Modification Enzymes and Complexes6. In Vitro Activity Assays for Myst Histone Acetyltransferases and Adaptation for High-Throughput Inhibitor ScreeningCheryl E. McCullough and Ronen Marmorstein7. Preparation and Biochemical Analysis of Classical Histone DeacetylasesAlejandro Villagra, Eva Sahakian and Edward Seto8. Recombinant Preparation, Biochemical Analysis, and Structure Determination of Sirtuin Family Histone/Protein DeacylasesBenjamin Suenkel and Clemens Steegborn9. Preparation, Biochemical Analysis and Structure Determination of SET Domain Histone MethyltransferasesElisa Bergamin and Jean Francois Couture10. Expression, Purification and Biochemical Analysis of the LSD1/KDM1A Histone DemethylaseBenoit Laurent and Yang Shi11. LSD1 Histone Demethylase Assays and InhibitionDawn Hayward and Philip A. Cole12. Purification, Biochemical Analysis, and Structure Determination of JmjC Lysine DemethylasesSwathi Krishnan and Raymond C. Trievel13. Preparation and Analysis of Native Chromatin Modifying ComplexesYannick Doyon and Jacques Côté
Histone Modification Binders (Readers)14. Preparation, Biochemical Analysis and Structure Determination of the Bromodomain, an Acetyl-lysine Binding DomainChunyan Ren, Lei Zeng and Ming-Ming Zhou15. Preparation, Biochemical Analysis and Structure Determination of Methyllysine ReadersCatherine A. Musselman and Tatiana G. Kutateladze
DNA Modifications and Nucleic Acid Regulators16. Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme ActivityMonica Yun Liu, Jamie E. DeNizio and Rahul M. Kohli17. Characterization of how DNA Modifications Affect DNA Binding by C2H2 Zinc Finger Proteins Anamika Patel, Hideharu Hashimoto, Xing Zhang and Xiaodong Cheng18. Crystallographic Studies of Telomerase Hunter Hoffman and Emmanuel Skordalakes19. Detection and Analysis of Long Non-Coding RNAsFan Lai, Ezra Blumenthal and Ramin Shiekhattar20. Identifying Centromeric RNAs Involved in Histone Dynamics in VivoDelphine Quénet, David Sturgill and Yamini Dalal
