Theory and Strategy in Histochemistry

1: General Considerations.- 1 The Scope of Histochemistry.- 2 The Structural and Chemical Basis for Histochemistry.- 3 Reagents.- 2: Review of Techniques According to Mechanism.- 4 General Theory for Tissue Staining.- 5 Blocking and Deblocking Reactions.- 6 Staining of Macromolecules on the Basis of Charge.- 7 Staining Involving Metal Complex Dyes.- 8 Staining Based on Reductants and Oxidants.- 9 Staining Involving Covalent Bonds.- 3: Tissue Processing.- 10 Tissue Processing I: Overview.- 11 Tissue Processing II: Freezing.- 12 Tissue Processing III: Fixation, General Aspects.- 13 Tissue Processing IV: Applied Fixation.- 14 Tissue Processing V: Embedding.- 15 Tissue Processing VI: Hard Tissues.- 16 Tissue Processing VII: Post Treatment.- 4: The Staining of Chemical Entities.- 17 Metals and Metal Salts.- 18 Pigments.- 19 Lipids.- 20 Nucleic Acids.- 21 Proteins.- 22 Carbohydrates.- 5: Enzyme Histochemistry.- 23 Enzyme Histochemistry I. General Considerations.- 24 Enzyme Histochemistry II. Hydrolases.- 25 Enzyme Histochemistry III. Oxidoreductases.- 6: Other Techniques.- 26 Immunohistochemistry.- 27 Ultrastructural Cytochemistry and Immunocytochemistry.- 28 Quantitation in Histochemistry.- 29 Autoradiography.- 30 Fluorescence Microscopic Methods in Histochemistry.- 7: An Introduction to Applied Histochemistry.- 31 Applied Histochemistry - An Overview.- 32 Applied Immunohistochemistry.- Appendix A: Standardization of Staining Methods.- A.I General Considerations.- A.2 Examples of Staining Methods.- Appendix B: Quantitative Methods in Microscopy.- B.I Definitions.- B.2 Observations.- B.3 Stereology.- B.4 Special Stereological Tools.- B.5 Simple "Counting" Procedures.- B.6 Manipulating Digital Images.- B.7 Applications of Stereology in Pathology.- References.- Index ofConstituents.- Index of Dyes.- Index of Methods.