This volume explores the latest methods used to study and define serpin molecular structure, basic protease inhibition, serpin targets, and the roles of serpin in biology and disease using animal models. The chapters in this book cover topics such as crystallography and phage display, peptide design, phospholipid binding, and thrombus formation to microbiome analysis and development. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and authoritative,
Serpins: Methods and Protocols
is a valuable resource for researchers and scientists interested in learning more about this evolving field.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
12
33 farbige Abbildungen, 12 s/w Abbildungen
XIII, 276 p. 45 illus., 33 illus. in color.
Dateigröße
ISBN-13
978-1-4939-8645-3 (9781493986453)
DOI
10.1007/978-1-4939-8645-3
Schweitzer Klassifikation
Overview of Serpins and Their Role in Biological Systems.- Methods for Determining and Understanding Serpin Structure and Function: X-Ray Crystallography.- Serpin Phage Display: Use of a T7 System to Probe Reactive Centre Loop Libraries with Different Serine Proteinases.- Kinetic Measurement of Serpin Inhibitory Activity by Real-Time Fluorogenic Biochemical Assay.- Methods for Identifying Virus-Derived Serpins.- In Vitro Approaches for the Assessment of Serpin Polymerization.- Cellular Models for the Serpinopathies.- Binding of Serpins to Immobilized Phospholipids and Phospholipids in Suspension.- Viral Serpin Reactive Center Loop (RCL) Peptides: Design and Testing.- In Vivo Analysis of Alpha 1 Antitrypsin Functions in Autoimmune Disease Models.- Analysis of In Vivo Serpin Functions in Models of Inflammatory Vascular Disease.- In Vivo Analysis of Alpha 1 Antitrypsin Functions in Autoimmune Disease Models.- Serpins in Venous Thrombosis and Venous Thrombus Resolution.- Next Generation Sequencing Library Preparation for 16S rRNA Microbiome Analysis after Serpin Treatment.- Methods for Assessing Serpins as Neuroprotective Therapeutics.- Serpins: Development as Clinical Therapeutics.- Serine Protease Assays: Measuring the Enzyme Targets for Serpins, Serine Protease Inhibitors.
