FOXO Transcription Factors

Introduction to FOXO Biology.- Characterization of microRNAs Regulating FOXO Expression.- Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter.- Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover.- Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity.- Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes.- Characterization of FOXO Acetylation.- Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays.- Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes.- Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters.- RNAi Mediated Silencing of FoxO Factors.- Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO.- High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO.- Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus.- Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology.- Genome-wide Analysis for Identifying FOXO Protein Binding Sites.- Mathematical Modeling of Nuclear Trafficking of FOXO Transcription Factors.- Following Transcriptome to Uncover FOXO Biological Functions.- Hydra as Model to Determine the Role of FoxO in Longevity.- Genetic Ablation of FOXO in Mice to Investigate its Physiological Role.- Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity.- Analysis of FOXO3a Gene Polymorphism Associated with Asthma.