Principles of Fluorescence Spectroscopy

1. Introduction to Fluorescence.- 2. Instrumentation for Fluorescence Spectroscopy.- 3. Fluorophores.- 4. Time-Domain Lifetime Measurements.- 5. Frequency-Domain Lifetime Measurements.- 6. Solvent Effects on Emission Spectra.- 7. Dynamics of Solvent and Spectral Relaxation.- 8. Quenching of Fluorescence.- 9. Advanced Topics in Fluorescence Quenching.- 10. Fluorescence Anisotropy.- 11. Time-Dependent Anisotropy Decays.- 12. Advanced Anisotropy Concepts.- 13. Energy Transfer.- 14. Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers.- 15. Energy Transfer to Multiple Acceptors, in One, Two, or Three Dimensions.- 16. Protein Fluorescence.- 17. Time-Resolved Protein Fluorescence.- 18. Excited-State Reactions.- 19. Fluorescence Sensing.- 20. Long-Lifetime Metal-Ligand Complexes.- 21. DNA Technology.- 22. Phase-Sensitive and Phase-Resolved Emission Spectra.- Appendix I. Corrected Emission Spectra.- 1. ?-Carboline Derivatives as Fluorescence Standards.- 2. Corrected Emission Spectra of 9,10-Diphenylanthracene, Quinine Sulfate, and Fluorescein.- 3. Long-Wavelength Standards.- 4. Ultraviolet Standards.- 5. Additional Corrected Emission Spectra.- References.- Appendix II. Fluorescent Lifetime Standards.- 1. Nanosecond Lifetime Standards.- 2. Picosecond Lifetime Standards.- 3. Representative Frequency-Domain Intensity Decays.- 4. Time-Domain Lifetime Standards.- References.- Appendix III. Additional Reading.- 1. Lifetime Measurements.- 2. Which Molecules are Fluorescent Representative Emission Spectra, and Practical Advice.- 3. Theory of Fluorescence and Photophysics.- 4. Principles of Fluorescence Spectroscopy.- 5. Biochemical Fluorescence.- 6. Protein Fluorescence.- 7. Data Analysis and Nonlinear Least Squares.- 8. Photochemistry.- 9. Flow Cytometry.-10. Phosphorescence.- 11. Polymer Science.- 12. Fluorescence Sensing.- 13. Immunoassays.- 14. Latest Applications of Fluorescence.- 15. Infrared and NIR Fluorescence.- 16. Lasers.- 17. Fluorescence Microscopy.- 18. Metal-Ligand Complexes and Unusual Luminophores.- Answers to Problems.