MicroRNA Technologies

Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord.- Protocol for High Content Screening for the Impact of Overexpressed MicroRNAs on Primary Motor Neurons.- Quantification of Dicer Activity in Mammalian Cell Lysates Using a Non-Radioactive Fluorescence Method.- Analysis of MicroRNAs and their Potential Targets in Human Embryonic Stem Cell-Derived Neurons Treated with the Anesthetic Protocol.- MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and Target Validation.- Study of miRNA Function in the Developing Axons of Mouse Cortical Neurons: Use of Compartmentalized Microfluidic Chambers and In Utero Electroporation.- Functional Analysis of Cortical Neuron Migration Using miRNA Silencing.- Continuous Delivery of Oligonucleotides into the Brain.- Isolating and Screening Subcellular miRNAs in Neuron.- Experimental Methods for Functional Studies in MicroRNAs in Animal Models of Psychiatric Disorders.- Isolation and Quantitative Analysis of Axonal Small Noncoding RNAs.- In Ovo Electroporation of miRNA Plasmids to Silence genes in a Temporally and Spatially Controlled Manner.- Three Dimensional Cell Culture of Human Neural Stem Cells using Polysaccharide-Based Hydrogels and Subsequent Bioanalyses.- Measuring miRNA Mediated Translational Regulation with Live Cell Imaging.