Basic Concepts in Clinical Biochemistry: A Practical Guide

Common clinical laboratory hazards and waste disposal.- Blood collection and preservation.- Quality Control in laboratory.- Automation in clinical laboratory.- Photometry-Colorimeter and Spectrophotometer.- Preparation of general laboratory solutions and buffers.- Examination of urine for normal constituents.- To perform qualitative tests for urinary proteins.- To determine the quantity of proteins in urine sample using Biuret reaction.- To estimate the amount of total protein and albumin in serum and to find A/G ratio.- To perform qualitative test for reducing substances in urine.- Quantitative analysis of reducing sugars in urine.- Estimation of blood glucose levels by Glucose Oxidase method.- Estimation of blood glucose levels by Folin and Wu method.- To perform Glucose Tolerance Test.- Estimation of urea in serum and urine.- To determine urea clearance.- To estimate creatinine level in serum and urine by Jaffe's reaction.- To determine creatinine clearance.- To determine the uric acid concentration in serum and urine.- Estimation of total calcium in serum and urine.- Estimation of inorganic phosphorus in serum and urine.- To estimate the amount of total cholesterol in serum.- To estimate total and direct bilirubin in serum.- To determine Alanine and Aspartate Transaminases activity in serum.- To estimate the activity of alkaline phosphatase in serum.- To estimate the activity of acid phosphatase in serum.- To determine serum and urinary amylase activity.- To estimate the activity of lipase in serum.- Qualitative analysis of ketone bodies in urine.- Qualitative test for bile pigments and urobilinogen in urine.- Determination of total lactate dehydrogenase activity in serum.- To measure the activity of Creatine kinase-MB and Total Creatine kinase in serum.- Analysis of Cerebrospinal Fluid for proteins and sugars.- To analyze lipid profile from given serum sample.- To determine sodium and potassium in serum by using Flame photometry.- To perform Radioimmunoassay.-To perform Enzyme linked Immunosorbant assay.- Some important case studies.