A Practical Guide to Pharmacological Biotechnology

Part1.Introduction.- 1.1 Rules of laboratory safety in a pharmacology laboratory.- 1.2 Commom instruments and their uses used in a pharmacology laboratory.- Part2.General aspects of Pharmacology laboratory.- 2.1 Safety and Risk assessment.- 2.2 Use and Handling of laboratory animals.- 2.3 Experimental design.- 2.4 Essential statistics.- 2.5 Cumulative Dose Response Curve.- 2.6 Toxicology.- 2.7 Basic instruments and techniques in Pharmacology Laboratory .- Part3.Isolated tissues and organs.- 3.1 Basic instruments used for isolated tissue experiments.- 3.2 Organ baths.- 3.3 Smooth muscle preparations.- 3.4 Skeletal muscle preparations.- 3.5 Cardiac muscle preparations.- Part4.Isolated tissues for screening of drugs.- 4.1 Evaluation of antidiabetic agents.- 4.2 Evaluation of antidepressants.- 4.3 Evaluation of antihypertensive agents.- 4.4 Evaluation of antiulcer agents.- 4.5 Evaluation of hepatotoxicity.- 4.6 Evaluation of Antioxidant agents.- 4.7 Evaluation of local anaesthetics.-Part5.Genotoxicity and Toxicological studies.- 5.1 The Mouse Lymphoma Assay.- 5.2 The Comet Assay.- 5.3 In vitro Genotoxicity assay.- 5.4 In vitro Teratogenicity Testing.- 5.5 Histopathological studies of animal tissues.- 5.6 Drug poisoning.- Part6.Experimental Animal studies.- 6.1 Pyrogen testing.- 6.2 Collecting blood from mice.- 6.3 Studies on different parameters of blood.- 6.4 Experiment on central nervous system.- 6.5 Evaluation on cardiovascular system.- 6.6 Experiments on GI tract.- Part7.Clinical trials.- 7.1 Clinical Pharmacology and its genesis.- 7.2 National and International agencies and their role in Clinical pharmacology.- 7.3 Stages in drug development and clinical trials.- 7.4 Ethics in Clinical research.- 7.5 Safety assessment in Clinical trials.- Part8.IPR and ethics in animal studies.- 8.1 Intellectual property rights and its different categories.- 8.2 Importance of IPR in drug development.- 8.3 Patenting cells, cell lines and animals.- 8.4 Ethics in laboratory animal studies .- 8.5 Risk assessment and management.- 8.6 Good laboratory practices.