CRISPR-Cas Systems for RNA and Genome Editing

1. Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sitesTJ Ha2. Genome oligopaint via local denaturation fluorescence in situ hybridizationTJ Ha3. TBDOsamu Nureki4. Biochemical reconstitution of a type I-B CRISPR-associated transposonLeifu Chang5. TBDYibei Xiao6. Visualising the conformational landscape of of CRISPR-Cas9 through kinetics-informed structural studiesDavid W. Taylor7. Analysis of metal-dependent DNA nicking activities by Cas endonucleasesDipali G. Sashital8. Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems.Ryan Jackson9. HYERJunjie (Gogo) liu10. focused on the purification and characterization of gRAMP, as well as its application in GFP knockdown in E. coli.Chunyi Hu11. TBDPeter Fineran12. Cas12a2 ?Chase Beisel13. Prime editing in bacteria with BacPEQuanjiang Ji14. TBDJia Chen15. Preparation of high-purity CRISPR-based DNA base editor RNPsAudrone Lapinaite16. Bacterial directed evolution of CRISPR base editorsShannon Miller17. Cloning and validating systems for high throughput molecular recordingMichelle Chan18. TBDSamagya Banskota19. Using CRISPR-Cas13 for viral nucleic acid detectionCameron Myhrvold20. CRISPR-based epigenetic editing for programmable gene silencing in mammalian cellsJames Nuñez21. Genome editing with programmable base editors in human cells" - explaining how to pick out and validate BE:gRNA combinations for a given target, with tips and tricks for maximizing editing efficienciesAlexis Komor22. High-throughput evaluation of genetic variants using base/prime editing sensor librariesFrancisco J. Sánchez Rivera23. TBDJia Chen