Spin Resonance Spectroscopy

Unit 1: Principle NMR and pulsed NMR Introduction - Spin Magnetic properties - Resonance condition - Larmor Frequency - NMR instrumentation - comparison of NMR & EPR - chemical shift - spin-spin coupling - Coupling constant - first and second order spectra - simplification of complex spectra - Boltzmann Statistics - Pulsed NMR - Basic principle of FT technique - Relaxation process - Bloch equations - Relaxation times - Line shape and line width analysis Unit 2: 1H and 13C NMR spectroscopy Interpretation of 1H NMR - assignment of signals - influence of factors on chemical shift of protons - 13C NMR - spin decoupling - double resonance - Nuclear Overhauser Effect - Off Resonance Decoupling - CIDNP - 13C assignment of signals - additivity rule - calculation of chemical shifts for aromatic and aliphatic compounds - 2D NMR -- DEPT 13C - 13C correlation COSY, HETCOR, NOE & NOSEY- multidimensional NMR Unit 3: Applications of NMRApplications of 31P, 19F and 15N - NMR spectroscopy used in structural problem - NMR of fluxional molecules - evaluation of rate constants - solid state NMR - magic angle spinning - NMR of paramagnetic molecules - contact shifts and shift reagents - NMR imaging - contrast agents Unit 4: EPR SpectroscopyPrinciple of EPR - instrumentation - total spin Hamiltonian - presentation of the spectrum - hyperfine splitting - super hyperfine structure - EPR of hydrogen atom - splitting in isotropic systems involving more than one nucleus - EPR spectra of free radicals in solution - methyl radical, benzene anion, p-benzosemiquinone radical anion, p- nitrobenzoate dianion and naphthalene anion - Evaluation of g and A tensors - factors affecting the magnitude of g values -- anisotropy - zero field splitting - Kramer's theory & degeneracy - triplet EPR Unit 5: Application and advanced EPR Application of EPR in transition metal complexes VO2+ Fe3+ Co2+ ,Mn2+, Ni2+ and bis-salicylaldimine copper (II) - Jahn-Teller theory & distortion studies in Cu(II) complexes - evaluation of spin - orbital coupling constant - exchange coupled EPR - multi resonance EPR - cw and pulsed EPR and ENDOR - MEMS and DAVIS - ESEEM - HYSCORE - ESR Spin trapping - spin labeling - EPR imaging