This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton's jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough,
Progenitor Cells: Methods and Protocols
is a valuable resource for scientists and researchers interested in learning more about this evolving and diverse field.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
57
11 s/w Abbildungen, 57 farbige Abbildungen
XIV, 289 p. 68 illus., 57 illus. in color.
Dateigröße
ISBN-13
978-1-4939-9631-5 (9781493996315)
DOI
10.1007/978-1-4939-9631-5
Schweitzer Klassifikation
