This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of
Staphylococcus aureus
in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant
S. aureus.
The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Informative and cutting-edge,
Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements,
Third Edition
is a valuable resource for researchers looking to set up new methods to study
S. aureus
, and will also be very useful for technicians and scientists working on other bacterial pathogens.
Reihe
Auflage
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
8 s/w Abbildungen, 27 farbige Abbildungen
XI, 268 p. 35 illus., 27 illus. in color.
ISBN-13
978-1-4939-9849-4 (9781493998494)
DOI
10.1007/978-1-4939-9849-4
Schweitzer Klassifikation
Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant
Staphylococcus aureus
Infections.- Rapid Methods for Detection of MRSA in Clinical Specimens.- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant
Staphylococcus aureus.-
Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Pulsed-Field Gel Electrophoresis Typing of
Staphylococcus aureus
Strains.- Spa Genotyping of
Staphylococcus aureus
Isolates.- Multilocus Sequence Typing of
Staphylococcus aureus.-
Genetic Manipulations of Staphylococcal Chromosomal DNA.- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology.- RNA-seq Identification of Target Genes Mediated by Regulators of
Staphylococcus aureus.-
Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of
Staphylococcus aureus.-
Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology.- Metabolomic Profiling of
Staphylococcus aureus.-
Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells.- Preclinical Models and Methodologies for Monitoring
Staphylococcus aureus
Infections using Noninvasive Optical Imaging.- Methicillin-Resistant
Staphylococcus aureus
Infection and Treatment Options.- Animal Models for Drug Development for MRSA.
