Protein Purification

Preface.
Contributors.
Part I. Introduction.
1.Introduction to Protein Purification (Bo Ersson, LarsRydén and Jan-Christer Janson).
Part II. Chromatography.
2. Introduction to Chromatography (Jan-Christer Janson andJan-Åke Jönsson).
3. Gel Filtration (Lars Hagel).
4. Ion Exchange Chromatography (Evert Karlsson and IrwinHirsh).
5. High-Resolution Reversed-Phase Chromatography (Sylvia WinkelPettersson).
6. Hydrophobic Interaction Chromatography (Kjell-Ove Erikssonand Makonnen Belew).
7. Immobilized Metal Ion Affinity Chromatography (LennartKågedal).
8. Covalent Chromatpgraphy (Francisco Batista-Viera, LarsRydén and Jan Carlsson).
9. Affinity Chromatography (Francisco Batista-Viera,Jan-Christer Janson and Jan Carlsson).
10. Affinity Ligands from Chemical Combinatorial Libraries(Enrique Carredano and Herbert Baumann).
11. Affinity Ligands from Biological Combinatorial Libraries(Per-Åke Nygren).
Part III. Other Separation Methods and RelatedTechniques.
12. Membrane Separations (Joachim K. Walter, Zuwei Jin, Maik W.Jornitz and Uwe Gottschalk).
13. Refolding of Inclusion Body Proteins from E. coli (ZhiguoSu, Siannan Lu and Zheng Liu).
14. Purification of PEGylated Proteins (Conan J. Fee and JamesM. Van Alstine).
Part IV. Electrophoresis.
15. Electrophoresis in Gels (Reiner Westermeier).
16. Conventional Isoelectric Focusing in Gel Slabs andCapillaries and Immobilized pH Gradients (Pier Giorgio Righetti,Alessia Farinazzo, Elisa Fasoli and Sabina Carla Righetti).
17. Two-Dimensional Electrophoresis in Proteomics (ReinerWestermeier and Angelika Görg).
18. Protein Elution and Blotting Techniques (ReinerWestermeier).
19. Capillary Electrophoretic Separations (WolfgangThormann).
Part V. Separation Method Optimization.
20. High Throughput Screening Techniques on Protein Purification(Karol M. Lacki and Eggert Brekkan).
Index.