TRNA-derived RNAs

1. Purification of endogenous tDRs by hybridization-based pulldownYasutoshi Akiyama2. Northern blotting for human pre-tRNA and tRNA-derived RNAsYasutoshi Akiyama3. In vitro translation systems to study tDRsPavel Ivanov4. tRNA derived RNAs that form tetramolecular assembliesPrakash Kharel5. Ribozyme-mediated expression of tRNA-derived small RNAs in bacteriaNorbert Polacek6. Ribosome-associated tDRs in yeastNorbert Polacek7. tDR bioinformaticsTodd Lowe8. cP-RNA-seq for the specific sequencing of tRNA halves and other cP-containing RNAsYohei Kirino9. TaqMan RT-qPCR for specific quantification of tRNA halves and other small RNAsYohei Kirino10. Detection and isolation extracellular tDRsAlfonso Cayota11. Preparation of nicked tRNAsJuan Pablo Tosar and Alfonso Cayota12. In vitro functional analysis of plant tDRsLaurence Drouard13. TGIRT-seq to profile tRNA-derived RNAs and associated RNA modificationsAnindya Dutta, Zhangli Su, Abigail Johnston and Monima Anam14. A general framework to over-express tRNA-derived RNAs from their parental tRNAs in mammalian cellsAnindya Dutta, Zhangli Su, Kumarjeet Banerjee and Fatemeh Esmaeili15. Functional characterization of tRNA-derived small RNAs in stem cellsCristian Bellodi16. Detection of mitochondrial tDRs in killifishClaire Riggs, Gazal Kalyan and Jason Podrabsky17. Measurement and comparison of full-length tRNA and tRF abundance and modificationsTao Pan and Luke Frietze18. Using a Fluorescence based tRNA to image tDR biogenesis and localization.Saumya Das and Guoping Li19. Manipulation of tDR expression using gain of function and loss of function approachesSaumya Das and Guoping Li20. TBDMark A. Kay and Hak Kyun Kim21. Nicked tRNAs in bacteriaSandra Wolin22. Determining Small RNA-Interacting Proteomes Using Endogenously Modified tRNA-Derived RNAsMatthias Ronald Schaefer, Vera Oberbauser and Aleksej Drino23. Activation of angiogenin by ribosome to produce tRNA-derived RNAsAndrei A. Korostelev and Anna B. Loveland