Ion Channels: Channel Production and Optical Methods

Preface Daniel L. Minor Jr. and Henry M. Colecraft Methods to produce and purify ion channels 1. Effective production of oligomeric membrane proteins by EarlyBac Hiro Furukawa 2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studies Lejla Zubcevic 3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy Studies Vera Moiseekova-Bell 4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM Studies Seok-Yong Lee 5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure Determination William A. Catterall 6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopy Nieng Yan 7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron Microscopy Show-Ling Shyng 8. Production of K2P2.1 (TREK-1) for structural studies Daniel L. Minor Jr. 9. Purification of a native nicotinic receptor Ryan Hibbs 10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs Stephen Brohawn Optical methods for probing and controlling channel function 11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino Acid Sebastian E. Brauchi 12. Fluorescence lifetime measurement of absolute membrane potential Evan Miller 13. Fluorescent Toxins as Ion Channel Activity Sensors Jon Sack 14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy Transfer Manu Ben-Johny 15. Manipulation of Ion Channel Gating with Photoswitchable Tweezers Thomas Grutter 16. Counting Subunits in Kv Channel Complexes Rikard Blunck