Ion Channels: Channel Chemical Biology, Engineering, and Physiological Function

Preface Daniel L. Minor Jr. and Henry M. Colecraft Methods to engineer ion channels to probe different aspects of channel structure-function and regulation. 1: Selection and validation of orthogonal tRNA / synthetase pairs for the encoding of unnatural amino acids across kingdoms. Christopher Ahern 2: Ion channel engineering using protein trans-splicing. Stephan Alexander Pless 3: ANAP: a versatile, fluorescent probe of ion channel gating and regulation. Michael C. Puljung 4: Engineering voltage sensing phosphatase, VSP. Yasushi Okamura 5: Probing Ion Channel Neighborhoods Using APEX. Steven Marx Methods to control and characterize ion channel function 6: Controlling ion channel trafficking by targeted ubiquination and deubiquitination. Henry M. Colecraft 7: Production of recombinant venom peptides as tools for ion channel research. Eitan Reuveny 8: Tethered Peptide Toxins for Ion Channels. Steven A. N. Goldstein 9: Stretch and poke stimulation for characterizing mechanically activated ion channels. Jörg Grandl 10: Optogenetic control of calcium influx in mammalian cells. YUBIN ZHOU 11: A method for assessing ionic and molecular permeation in connexin hemichannels. JORGE E. CONTRERAS Methods to characterize ion channel function and expression in physiology and disease 12: Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function. Polina V. Lishko 13: Live-cell measurements of individual melanosome pH using the novel ratiometric indicator RpHiMEL. Elena Oancea 14: Long Read Transcript Profiling of Ion Channel Splice Isoforms. Liz Tunbridge 15: Evaluating BEST1 Mutations in Pluripotent Stem Cell-derived Retinal Pigment Epithelial Cells. Tingting Yang 16: High Throughput Electrophysiological Evaluation of Mutant Ion Channels. Alfred L. George Jr. 17: In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy Nenad Bursac