Ion Channels: Channel Biochemistry, Reconstitution, and Function

Preface Daniel L. Minor Jr. and Henry M. Colecraft Structure/function relationships 1. Correlating ion channel structure and function Crina Nimigean 2. TRPV3 Expression and Purification for Structure Determination by Cryo-EM Alexander Sobolevsky 3. Methods to study phosphoinositide regulation of ion channels Tibor Rohacs 4. Recombinant Expression and Purification of Pentameric Ligand-Gated Ion Channels for Cryo-EM Structural Studies Sudha Chakarpani 5. Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography Anna Moroni Structural and chemical methods to assess channel dynamics 6. Structural Dynamics of Channels and Transporters by High-Speed Atomic Force Microscopy Simon Scheuring 7. Crosslinking for gating motions in GluRs Andrew Plested 8. Single molecule FRET methodology for investigating glutamate receptors Vasanthi Jayaraman 9. Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues Murali Prakriya Channel reconstitution and studies in bilayers 10. A quantitative flux assay for the study of Cl- channels and transporters Alessio Accardi 11. Ion channels reconstitution in lipid bilayers Eleonora Zakharian 12. Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers Gehard Thiel Methods to assess oligomerization 13. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture Randy Stockbridge