Integrated Methods in Protein Biochemistry: Part A

1. DNA and protein engineering to create protein bioswitches with new functionsStewart Loh2. Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteasesSusan Michaelis3. An experimental protocol to study lipid transfer proteinsHaijia Yu4. Synthesis of small heat shock proteinsMatthew Pratt5. Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteinsGuang-Yu Yang6. An experimental protocol for glycoconjugate analysisYifa Zhou7. Methods for proximity-based biotinylation combined with Mass SpectrometryRalph Kehlenbach8. Synthetic antibody fragments as conformational sensors of protein activation and traffickingArun K. Shukla9. Expression, purification, functional analysis and crystallization of Rag GTPaseKuang Shen10. Purification of bacterial transcription elongation complexes by photoreversible immobilizationEric Strobel11. Inhibition of c-Myc-MAX heterodimerizationNeha Garg12. Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerasesMarkus C. Wahl13. Phosphorylation and subcellular localization of human phospholipase A1Atsushi Yamashita14. Integrated methods in studying light-sensitive proteinsLars-Oliver Essen15. Structure and biochemistry of thermophilic proteinAnkan Banerjee16. Using hydrogen/deuterium exchange mass spectrometry to probe protein structureGert Bange17. Nanopore force spectroscopy to probe structural dynamics of proteinsSonja Schmid18. Structural analysis of knotted proteinsShang-Te "Danny" Hsu19. Mutation-induced structural changes in the SARC-CoV2 spike proteinShang-Te "Danny" Hsu20. Preparing metabolite capped RNA substrates using RNA polymerasesJeremy G. Bird