Molecular Neurobiology

1. The Molecular Biology of the Na,K-ATPase and Other Genes Involved in the Ouabain-Resistant Phenotype.- 1. Introduction.- 2. Molecular Cloning of the Na,K-ATPase Catalytic Subunit.- 3. Organization and Expression of the Rat Sodium Pump a-Subunit Gene.- 4. Organization and Expression of the a-Subunit Gene in Ouabain-Resistant Cell Lines.- 5. Isolation and Characterization of a Ouabain-Resistance Gene.- 6. Transfer of the Sodium Pump a-Subunit Gene Confers Ouabain Resistance to Ouabain-Sensitive Cells.- 7. Isolation of Genes Related to the Sodium Pump and Their Expression in a Ouabain-Resistant Cell Line.- 8. Conclusions and Future Prospects.- References.- 2. Molecular Biology of the Genes Encoding the Major Myelin Proteins.- 1. Introduction.- 2. Formation and Structure of the Myelin Sheath.- 3. Myelin-Specific Proteins.- 4. Conclusion.- References.- 3. Molecular Biology of the Neural and Muscle Nicotinic Acetylcholine Receptors.- 1. Introduction.- 2. Isolation of cDNA Clones Coding for the Acetylcholine Receptor Expressed in the Torpedo Electric Organ.- 3. Isolation of cDNA Clones Coding for Mouse Skeletal Muscle Acetylcholine Receptor.- 4. Structure of the Acetylcholine Receptor.- 5. Expression of the Acetylcholine Receptor Genes in Skeletal Muscle.- 6. Brain Receptors.- 7. Conclusion.- References.- 4. Molecular Biology of Muscle Development: The Myosin Gene Family of Caenorhabditis elegans.- 1. Introduction.- 2. Genetics of the unc-54 Locus.- 3. Immunological Identification and Localization of Myosin Isoforms.- 4. Molecular Cloning of the unc-54 and myo-1,2,3 MHC Genes.- 5. Immunological Identification of the Products of the myo-1,2,3 MHC Genes.- 6. Structural Organization of the Nematode MHC Genes.- 7. Molecular Anatomy of the Myosin Molecule.- 8. Sequences and Molecular Interpretation of unc-54 Mutations.- 9. Myosin Protein Expression and Mutagenesis in E. coli.- References.- 5. Small Cardioactive Peptides in A and B: Chemical Messengers in the Aplysia Nervous System.- 1. Introduction.- 2. The Distribution of SCP-Immunoreactive Neurons in the CNS.- 3. The SCP Gene and Precursor Protein.- 4. SCP Gene Expression.- 5. Subcellular Localization.- 6. Coexistence of Multiple Transmitters.- 7. Physiological Activities.- 8. Conclusions.- References.- 6. Molecular Biology Approach to the Expression and Properties of Mammalian Cholinesterases.- 1. Introduction: Expression of Cholinesterases as a Research Subject-Scientific Significance, Advantages, and Difficulties.- 2. Expression of Cholinesterase mRNAs in Microinjected Xenopus Oocytes.- 3. Identification of Drosophila DNA Fragment that Hybridizes with Cholinesterase mRNA.- 4. Isolation and Partial Characterization of Human DNA Fragments Homologous to DroSR.- 5. Preparation of Synthetic Oligonucleotide Probes According to the Consensus Sequence at the Organophosphate-Binding Site.- 6. Preliminary Characterization of Neuroche cDNA Clones.- 7. Summary and Conclusions.- References.- 7. Genes and Gene Families Related to Immunoglobulin Genes.- 1. Introduction.- 2. Immunoglobulin Genes and the Immunoglobulin Domain.- 3. The T-Lymphocyte Cell-Surface Receptor for Antigen.- 4. Class I MHC Genes.- 5. Class II MHC Genes.- 6. Cell-Surface Receptors for Transepithelial Transport of Immunoglobulin.- 7. The T-Cell Accessory Molecules T4 and T8.- 8. Related Members of the Immunoglobulin Supergene Family Expressed in the Nervous System.- 9. Conclusion: The Evolution of the Immunoglobulin Super family..- References.- 8. Specificity of Prohormone Processing: The Promise of Molecular Biology.- 1.Introduction.- 2. Gene-Transfer Systems.- 3. Enzymatic Studies.- 4. The Internal Environment of Secretory Granules.- 5. Perspectives.- References.