Studying the Pro-Migratory Effects of MIF.- In Vitro Approaches for Investigating the Influence of MIF on Leukocyte-Endothelial Cell Interactions under Flow Conditions.- Using Intravital Microscopy to Study the Role of MIF in Leukocyte Trafficking In Vivo.- Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells.- Measuring MIF in Biological Fluids.- Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wildtype and MIF KO Mice.- Genotyping Two Promoter Polymorphisms in the MIF Gene: a -794 CATT5-8 Microsatellite Repeat and a -173 G/C SNP.- Staining MIF in Cells for Confocal Microscopy.- Microscopy Methods for Imaging MIF and its Interaction Partners.- Co-Immunoprecipitation (Co-IP) of Macrophage Migration Inhibitory Factor (MIF).- Concurrent Immunohistochemical Localization and Western Blot Analysis of the MIF Receptor, CD74, in Formalin-Fixed, Paraffin-Embedded Tissue.- Methods to Determine the Effects of MIF on In Vitro Osteoclastogenesis using Murine Bone Marrow-Derived Cells and Human Peripheral Blood Mononuclear Cells (PBMCs).- Inducing and Inhibiting Autophagy to Investigate its Interactions with MIF.- Assays for Measuring the Role of MIF in NLRP3 Inflammasome Activation.- Assays for Inducing and Measuring Cell Death to Detect Macrophage Migration Inhibitory Factor (MIF) Release.- Assessing the Role of MIF in Plasmodium spp. Infections using Ex Vivo Models.- The Effect of Macrophage Migration Inhibitory Factor on Intestinal Permeability: FITC-Dextran Serum Measurement and Transmission Electron Microscopy.- Investigating MIF in Mouse Models of Severe Corticosteroid-Resistant Neutrophilic Asthma.- Investigating MIF in Mouse Model of Gout.- 5´and 3' RACE Method to Obtain Full-Length 5' and 3' Ends of Ciona robusta Macrophage Migration Inhibitory Factors MIF 1 and MIF 2 cDNA.- Purification of Antibodies against Entamoeba histolytica MIF and their use in Analyzing Human and Mouse Samples.- Studying Plant MIF/D-DT-Like Genes and Proteins (MDLs).
