This volume explores the latest techniques and protocols used by researchers to address unique biological questions, model organisms not typically studied by Fluorescent In Situ Hybridization (FISH), protocols combining FISH with immunofluorescence (FISH-IF), and high-throughput experiments. The chapters in this book are divided into two parts: RNA FISH protocols and DNA FISH protocols. Part One covers methods for designing OligoPaint probes and studying distinct aspects of RNA biology such as transcription and splicing dynamics, and mRNA and small RNA expression and localization. Part Two discusses DNA repair dynamics, gene compaction, and chromatin conformation and gene rearrangements in plants, insects, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Fluorescence In Situ Hybridization (FISH): Methods and Protocols is a valuable resource that will benefit the broader scientific community in their studies and understanding of this important field.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
67
1 s/w Abbildung, 67 farbige Abbildungen
XII, 302 p. 68 illus., 67 illus. in color.
Dateigröße
ISBN-13
978-1-0716-3766-1 (9781071637661)
DOI
10.1007/978-1-0716-3766-1
Schweitzer Klassifikation
Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria.- Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in the Fungal Pathogen Candida albicans.- RNA and Protein Detection by Single-Molecule Fluorescent In Situ Hybridization (smFISH) Combined with Immunofluorescence in the Budding Yeast S. cerevisiae.- Fluorescence In Situ Hybridization as a Tool for Studying the Specification and Differentiation of Cell Types in Nematostella vectensis.- SABER-FISH in Hydractinia.- smFISH for Plants.- Fluorescent In Situ Detection of Small RNAs in Plants using sRNA-FISH.- hcHCR: High-Throughput Single-Cell Imaging of RNA in Human Primary Immune Cells.- High-Throughput RNA-HCR-FISH Detection of Endogenous Pre-mRNA Splice Variants.- Simultaneous In Situ Detection of m6A-Modified and Unmodified RNAs using DART-FISH.- Multiplexed Immunofluorescence and Single Molecule RNA Fluorescence InSitu Hybridization in Mouse Skeletal Myofibers.- Designing Oligonucleotide-Based FISH Probe Sets with PaintSHOP.- Efficient Prediction Model of mRNA End-to-End Distance and Conformation: Three-Dimensional RNA Illustration Program (TRIP).- Combined 3D DNA FISH, Single Molecule RNA FISH, and Immunofluorescence.- Determining the Compaction State of Genes using DNA FISH.- Hi-M: A Multiplex Oligopaint FISH Method to Capture Chromatin Conformations In Situ and Accompanying Open-Source Acquisition Software.- Rapid DNA-FISH in Arabidopsis thaliana Somatic Cells.- DBD-FISH Using Specific Chromosomal Region Probes for the Study of Cervical Carcinoma Progression.- CRISPR-Based Split Luciferase as a Biosensor for Unique DNA Sequences In Situ.
