Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics.
* Is the only current book available on the subject
* Includes author insights and practical tips
* Is an ideal single source for students and researchers working in proteomics
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Techn.
ISBN-13
978-0-08-053362-9 (9780080533629)
Schweitzer Klassifikation
Preface.Incidents of Travel in IEF and IPGs.Overview.Evolution and Development of IEP.Theory and Simulation of IEF.Generation of pH Gradients. Slab Gel IEF. Two-Dimensional Gel Electrophoresis.Some Practices and Pitfalls of Sample Preparation for IEF in Proeomics.Protein Detection and Imaging in IEF Gels. Capillary IEF.Free Flow IEF.Isoelectric Focusing and Proteomics.Chromatofocusing.Alternative Forms of IEF.
