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This book serves as a practical guide and a manual on the application of isotopic tracers in understanding the cellular processes (e.g. molecular interactions, protein synthesis) in growth, development, and disease conditions. The techniques described in the book have been carefully chosen to underscore the role and relevance of isotopic tracers in the identification of molecular targets and mechanisms, and preclinical validation of potential therapeutics. This book is intended to be a valuable resource to non-experts that may include students, researchers, educators/teachers, and others who have an interest to understand the application of isotopic tracers in research. Each chapter includes a background, an outline of the method and underlying principles, and a detailed step-wise protocol. To augment the clarity and intellectual grasp, the chapters also include relevant illustrations and study questions. More importantly, the book covers techniques involving radioactive as well asnon-radioactive tracers to expand the scope of isotopes in preclinical research including basic and applied science.
Shanmugasundaram Ganapathy-Kanniappan holds a Ph.D. degree from the University of Madras, India. In 2006, he joined the Department of Radiology, Johns Hopkins University School of Medicine, as a junior faculty member and was quickly promoted to assistant professor. Before joining the Johns Hopkins, he underwent postdoctoral training in premier institutes such as the National Institute of Immunology (NII, New Delhi, India) and the University of California at Los Angeles (UCLA). His current research interests include metabolic alteration in human pathophysiology and the identification of molecular targets for therapeutic intervention. He has published over 50 articles including original reports, invited reviews, editorials, book chapters, and books (https://www.ncbi.nlm.nih.gov/myncbi/1xKsymd-il85x/bibliography/public/). He is an inventor and coinventor of several patents and a recipient of prestigious fellowships and research awards including funding from private and federal sponsors. He is on the editorial board of peer-reviewed journals and currently serves the journal, Frontiers in Oncology as an associate editor for the section, Cancer Molecular Targets and Therapeutics.
1. Introduction.- 2. Tracer technique in basic research.- 3. Tracer technique in applied research.- 4. Literature cited
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