Tumor Immunology and Immunotherapy - Molecular Methods

1. Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsies Sara Valpione and Luca Campana 2. Detection and quantification of cytosolic DNA Sheila Spada, Takahiro Yamazaki and Claire Vanpouille-Box 3. Methods to detect endogenous dsRNA induction and recognition Ilias Ettayebi, Helen Loo Yau and Daniel D. De Carvalho 4. Quantification of eIF2alpha phosphorylation during immunogenic cell death Lucillia Bezu, Alejandra Wu Chuang, Juliette Humeau, Guido Kroemer and Oliver Kepp 5. Assessment of annexin A1 release during immunogenic cell death Elisa Elena Baracco, Adriana Petrazzuolo and Guido Kroemer 6. Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells George R. Dubyak 7. Quinacrine-mediated detection of intracellular ATP Sabrina Forveille, Juliette Humeau, Allan Sauvat, Lucillia Bezu, Guido Kroemer and Oliver Kepp 8. Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells Francesco Di Virgilio, Lin-Hua Jiang, Sèbastien Roger, Simonetta Falzoni, Alba Clara Sarti, Valentina Vultaggio-Poma, Paola Chiozzi and Elena Adinolfi 9. LEGENDplex(TM): Bead-assisted multiplex cytokine profiling by flow cytometry Jason S. Lehmann, Priyanka Rughwani, Melissa Kolenovic, Shaoquan Ji and Binggang Sun 10. Methods for measuring HMGB1 release during immunogenic cell death Liwei Zhao, Peng Liu, Oliver Kepp and Guido Kroemer 11. Different measures of HMGB1 location in cancer immunology Carolina Mendonça Gorgulho, Pranav Murthy, Lance Liotta, Virginia Espina and Michael T. Lotze 12. Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissues Sunil Thomas, Lisa Laury-Kleintop and George C. Prendergast 13. Quantification of IDO1 enzyme activity in normal and malignant tissues Lijie Zhai, Erik Ladomersky, April Bell, Corey Dussold, Krislyn Cardoza, Jun Qian, Kristen L. Lauing and Derek A. Wainwright 14. Enzyme activity of circulating CD73 in human serum Silvana Morello, Roberta Turiello, Gabriele Madonna, Aldo Pinto, Paolo A. Ascierto andMariaelena Capone 15. Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assays Bertrand Allard, Isabelle Cousineau, Kathleen Spring and John Stagg 16. Distinguishing perforin-mediated lysis and granzyme-dependent apoptosis Jesse A. Rudd-Schmidt, Joseph A. Trapani and Ilia Voskoboinik 17. Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterization Leepakshi Khurana, Mei ElGindi, Pathricia V. Tilstam and Georgios Pantouris 18. In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1 Wenjie Zhai, Xiuman Zhou, Jiangfeng Du and Yanfeng Gao 19. Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodies Norimichi Nomura, Yayoi Nomura, Yumi Sato and So Iwata 20. High-throughput discovery of cancer-targeting TCRs Lukas Bunse, Edward W. Green and Michael Platten 21. TCR validation toward gene therapy for cancer Edward W. Green, Lukas Bunse, Matthias Bozza, Khwab Sanghvi and Michael Platten 22. Analysis of TCR ß CDR3 sequencing data for tracking anti-tumor immunity Jiajia Zhang, Zhicheng Ji and Kellie N. Smith 23. Quantitative analysis of the T cell receptor repertoire Imran Uddin, Annemarie Woolston, Thomas Peacock, Kroopa Joshi, Mazlina Ismail, Tahel Ronel, Connor Husovsky and Benny Chain 24. CRATES: A one-step assembly method for Class 2 CRISPR arrays Chunyu Liao, Rebecca A. Slotkowski and Chase L. Beisel