K+ channels regulate the passage of potassium ions across lipid membranes, influencing numerous biological functions, including homeostasis, sensory perception, and information transmission. This volume explores the widespread involvement of K+ channels in cellular processes, which makes these membrane proteins intriguing targets for both fundamental scientific inquiry and the development of pharmaceutical compounds. The chapters in this book provide an in-depth look at various techniques and the latest protocols commonly used in ion channel research to investigate K+ channel structures, functions, and their interactions with drugs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Potassium Channels: Methods and Protocols is a valuable resource for any researcher who is interested in learning more about potassium channels. Chapters 5 and 7 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
10
63 farbige Abbildungen, 10 s/w Abbildungen
XI, 292 p. 73 illus., 63 illus. in color.
Dateigröße
ISBN-13
978-1-0716-3818-7 (9781071638187)
DOI
10.1007/978-1-0716-3818-7
Schweitzer Klassifikation
Asymmetric Lipid Bilayers and Potassium Channels Embedded therein in the Contact Bubble Bilayer.- Detecting Bound Ions in Ion Channels by Solid-State NMR Experiments on 15N Labelled Ammonium Ions.- Conformational Dynamic Studies of Prokaryotic Potassium Channels Explored by Homo-FRET Methodologies.- Purification of Potassium Ion Channels Using Styrene-Maleic Acid Copolymers.- Ready to Record Cells for Kinetic Screening of VGICs.- Cell-Based Thallium-Influx Fluorescence Assay for Kv10.1 Channels.- Use of Surface Plasmon Resonance Technique for Studies of Inter-Domain Interactions in Ion Channels.- Voltage Clamp Fluorometry: Illuminating the Dynamics of Ion Channels.- Creating Computational Models of Ion Channel Dynamics.- Human Kir2.1 Potassium Channel: Protocols for Cryo-EM Data Processing and Molecular Dynamics Simulations.- Evaluation of the Role of Potassium Channels in the Proliferation, Migration, and Invasion of Blood Cells.- Non-Radioactive Rb+ Efflux Assay for Screening KATP Channel Modulators.- The Dynamic Clamp Technique: A Robust Toolkit for Investigating Potassium Channel Function.- Automated Patch Clamp Recordings of GPCR-Gated Ion Channels: Targeting the MC4-R/Kir7.1 Potassium Channel Complex.- Machine Learning Methods for the Analysis of the Patch-Clamp Signals.- Isothermal Titration Calorimetry for Fragment-Based Analysis of Ion Channel Interactions.
