RNA Methodologies

1. RNA and the cellular biochemistry revisited 2. Creating a ribonuclease-free environment 0003. Stringency: conditions that influence nucleic acid structure4. Ribonucleic acid isolation strategies5. Isolation of polyadenylated RNA6. The truth about tissues7. Going green: RNA and the molecular biology of plants8. Quality control for RNA preparations9. cDNA: a permanent biochemical record of the cell10. RT-PCR: a science and an art form11. Quantitative polymerase chain reaction techniques12. miRNA and other noncoding RNAs13. RNA interference and gene editing14. Electrophoresis of RNA15. Photodocumentation and image analysis16. Northern analysis17. Nucleic acid probe technology18. Quantification of specific mRNAs by nuclease protection19. Analysis of nuclear RNA20. RNA in situ hybridization21. Array analysis of gene expression22. Subtractive and nonsubtractive methods for the analysis of gene expression23. Transcriptomes and bioinformatics24. RNA-seq: the premier transcriptomics tool25. RNA biomarker discovery and validation26. Functional genomics strategies27. A few RNA success stories EpilogueAppendix A: Maintaining complete and accurate records Appendix B: Converting mass to moles Appendix C: Removal of DNA from an RNA sample Appendix D: Removal of RNA from a DNA sample Appendix E: Electrophoresis: principles, parameters, and safety Appendix F: Disposal of ethidium bromide and SYBR Green solutions Appendix G: Deionization of formamide, formaldehyde, and glyoxal Appendix H: Silanizing centrifuge tubes and glassware Appendix I: Centrifugation as a mainstream tool for the molecular biologist Appendix J: Dot blot analysis Appendix K: Useful stock solutions for the molecular biologist Appendix L: Genomes and proteomes Appendix M: Common SI prefixes Appendix N: Common abbreviations Appendix O: Select suppliers of equipment, reagents, and services Appendix P: Trademark citations Glossary