Isotachophoresis

Name: Isotachophoresis | Theory, Instrumentation and Applications
Brand: Elsevier
Price: 240.0 EUR
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Theory, Instrumentation and Applications

F. M. Everaerts J. L. Beckers T. P. E. M. Verheggen(Herausgeber*in)

Elsevier (Verlag)

Erschienen am 22. September 2011

417 Seiten

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978-0-08-085806-7 (ISBN)

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Inhalt

Front Cover
Isotachophoresis: Theory, Instrumentation and Applications
Copyright Page
Contents
Preface
Chapter 1. Historical review
Summary
1. Historical review
References
PART 1: THEORY
Chapter 2. Principles of electrophoretic techniques
Summary
2.1. Introduction
2.2. Principle of zone electrophoresis
2.3. Principle of moving-boundary electrophoresis
2.4. Principle of isotachophoresis
2.5. Principle of isoelectric focusing
2.6. Discussicin
Chapter 3. Concept of mobility
Summary
3.1. Introduction
3.2. Interpretation of electrophoretic migration
3.3. Ionic mobility and ionic equivalent conductivity
3.4. Effective ionic mobility
3.5. Determination of ionic mobilities
References
Chapter 4. Mathematical model for isotachophoresis
Summary
4.1. Introduction
4.2. General equations
4.3. Mathematical model for the steady state in isotachophoresis
4.4. Validity of the isotachophoretic model
4.5. Check of the isotachophoretic model
References
Chapter 5. Choice of electrolyte systems
Summary
5.1. Introduction
5.2. Choice of the solvent
5.3. Choice of the buffering counter ionic species
5.4. Choice of the pH of the leading electrolyte
5.5. Choice of the terminating and leading ionic species
5.6. Additions to the electrolyte solutions
5.7. Discussion
5.8. Examples
References
PART 2: INSTRUMENTATION
Chapter 6. Detection systems
Summary
6.1. Introduction
6.2. Thermometric recording
6.3. High-frequency conductivity detection
6.4. Conductivity detection
6.5. UV absorption meter
6.6. Additives to the electrolytes
6.7. Coating of the micro-sensing electrodes
6.8. Detection limits
6.9. Conclusion
References
Chapter 7. Instrumentation
Summary
7.1. Introduction
7.2. Injection systems
7.3. Counter electrode compartments
7.4. Equipment
7.5. Counter flow of electrolyte
PART 3: APPLICATIONS
Chapter 8. Introduction
Summary
8. Introduction
Chapter 9. Practical aspects
Summary
9.1. Introduction
9.2. Disturbances caused by hydrogen and hydroxyl ions
9.3. Disturbances due to the presence of carbon dioxide
9.4. Enforced isotachophoresis
9.5. Water as terminator
9.6. Purification of the terminator
9.7. Conversion of data measured with different detectors
References
Chapter 10. Quantitative aspects
Summary
10.1. Introduction
10.2. Theoretical
10.3. Thermometric measurements
10.4. Conductimetric measurements
10.5. Conclusion
References
Chapter 11. Separation of cationic species in aqueous solutions
Summary
11.1. Separation of cationic species in aqueous solutions using a thermocouple as detector
11.2. Separation of cationic species in water and deuterium oxide using a conductivity detector (a.c. method) and a UV absorption detector (256 nm)
Chapter 12 . Separation of anionic species in aqueous solutions
Summary
12.1. Separation of anionic species in aqueous solutions using a thermometric detector
12.2. Separation of anionic species in aqueous solutions using a conductivity detector (a.c. method) and a UV absorption detector (256 nm)
Chapter 13. Amino acids, peptides and proteins
Summary
13.1. Amino acids
13.2. Separation of proteins in ampholyte gradients
13.3. Separation of small peptides
References
Chapter 14. Separation of nucleotides in aqueous systems
Summary
14.1. Introduction
14.2. Separation using a thermometric detector
14.3. Separation using a conductivity detector (a.c. method) and a UV absorption detector (256 nm)
Chapter 15. Enzymatic reactions
Summary
15.1. Introduction
15.2. Enzymatic conversion of glucose (fructose) into glucose-6-phosphate (fructose-6-phosphate) with hexokinase from yeast
15.3. Enzymatic conversion of pyruvate into lactate with lactate dehydrogenase from pig heart
References
Chapter 16. Separations in non-aqueous systems
Summary
16.1. Introduction
16.2. Separation of anionic species in methanol using a thermometric detector
16.3. Separation of cationic species in methanol using a thermometric detector
16.4. Experiments in aqueous methanolic systems using a conductimetric detector (a.c. method) and UV absorption detector (256 nm)
Chapter 17. Counter flow of electrolyte
Summary
17.1. lntroduction
17.2. Experimental
17.3. Conclusion
References
PART 4: APPENDICES
Appendix A. Simplified model of moving-boundary electrophoresis for the measurement of effective mobilities
A.1. Introduction
A.2. Model of moving-boundary electrophoresis
A.3. Procedure of computation
A.4. Experimental
A.5. Discussion
References
Appendix B. Diameter of the narrow-bore tube, applied for separation
Appendix C. Literature
Symbols and abbreviations
Symbols
Subscripts
Superscripts
Examples
Abbreviations
Subject index

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