The Enzymes

Name: The Enzymes
Brand: Academic Press
Price: 54.95 EUR
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Academic Press

3. Auflage

Erschienen am 22. März 1982

641 Seiten

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Inhalt

Front Cover
The Enzymes: Nucleic Acids
Copyright Page
Contents
List of Contributors
Preface
Section I. Ligases
Chapter 1. DNA Ligases
I. Introduction
II. Isolation and Physical Properties
III. Catalytic Properties
IV. Role of DNA Ligases in Vivo
V. Research Applications
Chapter 2. T4 RNA Ligase
I. Introduction
II. Purification and Properties
III. Reactions Catalyzed
IV. Biological Role
V. Applications
Section II. RNA Polymerares and Related Enzymes
Chapter 3. Bacterial DNA-Dependent RNA Polymerases
I. Background
II. Molecular Properties
III. Catalytic Properties
Chapter 4. Bacteriophage DNA-Dependent RNA Polymerases
I. Introduction
II. T7-Like RNA Polymerases
III. Other Bacteriophage RNA Polymerases
Chapter 5. Eukaryotic RNA Polymerases
I. Introduction
II. Purification
III. Subunit Structures
IV. Subunit Functions
V. Stimulatory Factors
VI. DNA Binding and Catalytic Properties of Purified RNA Polymerases
VII. Inhibitors
VIII. Eukaryotic Transcription Extract Systems
IX. Organelle- and Viral-Coded RNA Polymerases
Chapter 6. Priming Enzymes
I. Introduction
II. dnaG Gene Product from Escherichia coli
III. Multiple Pathways for dnaG Priming in SS to RF DNA Replication
IV. Studies on the Specificity of dnaG-Template Interactions
V. Priming by RNA Polymerases
VI. Priming on Double-Stranded DNA
VII. Phage-Encoded Priming Enzymes
VIII. Priming in Eukaryotic Systems
Chapter 7. tRNA Nucleotidyltransferase
I. Introduction
II. Purification and Structural Studies
III. Catalytic Properties
IV. Biological Role
V. Research Applications
Chapter 8. Poly(A) Adding Enzymes
I. Introduction and Perspective
II. Purification and Properties
III. Multiple Poly(A) Polymerases
IV. Properties of Poly(A) Polymerase Proteins
V. Assay
VI. Stoichiometry
VII. Substrates
VIII. Primer Requirement
IX. Ion Requirements
X. Inhibitors
XI. Kinetics and Reaction Mechanism
XII. Biological Role
XIII. Regulation of Poly(A) Polymerases
XIV. Research Applications
Chapter 9. Capping Enzyme
I. Introduction
II. Vaccinia Virus Capping Enzymes
III. HeLa Cell Capping Enzymes
IV. Capping Enzyme from Rat Liver Nuclei
V. Role of the Capping Enzyme System in Vivo
VI. Research Applications of Vaccinia Virus Capping Enzyme
Chapter 10. Qß Replicase
I. Introduction
II. Purification and Properties
III. Catalytic Properties
Chapter 11. 2',5'-Oligoadenylate Synthetase
I. Introduction
II. Purification and Properties
III. Reactions Catalyzed
IV. Biological Role
Section III. RNA Nucleases and Related Enzymes
Chapter 12. Pancreatic Ribonuclease
I. Introduction
II. Preparation
III. Chemical Properties
IV. Physical Properties
V. Species Variations
VI. Bovine Seminal Plasma RNase
VII. Cytoplasmic RNase Inhibitor
VIII. Catalytic Properties
IX. Research Applications
Chapter 13. Ribonuclease T1
I. Introduction
II. Purification and Chemical Properties
III. Reactions Catalyzed
IV. Research Applications
V. Other Guanine-Specific RNases
Chapter 14. tRNA Processing Enzymes from Escherichia coli
I. Introduction
II. Ribonuclease P
III. Ribonuclease III
IV. Ribonuclease P2 and Ribonuclease O
V. Ribonuclease D
VI. Other Nucleases
VII. Concluding Remarks
Chapter 15. Ribonuclease III
I. Introduction
II. Purification
III. Structure
IV. Cleavage of Double-Stranded RNA
V. Cleavage of Single-Stranded RNA
VI. Related Enzymes from Eukaryotes
Chapter 16. RNases I, II, and IV of Escherichia coli
I. Introduction
II. RNase I of Escherichia coli
III. RNase II of Escherichia coli
IV. RNase IV of Escherichia coli
Chapter 17. Polynucleotide Phosphorylase
I. Introduction
II. Properties
III. The Reactions Catalyzed
IV. Attributed Physiological Functions
V. Research Applications
Section IV. RNA Modification
Chapter 18. RNA Methylation
I. Introduction
II. Structures and General Assay Procedure
III. Specific tRNA Methyltransferase Enzymes
IV. Conclusion
Chapter 19. Nucleotide Modification in RNA
I. Introduction
II. Modification of Uridine
III. Modification of Cytidine
IV. Modification of Adenosine
V. Modification of Guanosine
VI. Conclusion
Author lndex
Subject Index
Contents of Other Volumes

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