This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Stable Isotope Probing: Methods and Protocols
provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
35 farbige Abbildungen, 8 s/w Abbildungen
XI, 247 p. 43 illus., 35 illus. in color.
Dateigröße
ISBN-13
978-1-4939-9721-3 (9781493997213)
DOI
10.1007/978-1-4939-9721-3
Schweitzer Klassifikation
