Mass Spectrometry of Protein Interactions

Preface.
Contributors.
Chapter 1. Direct Characterization of Protein Complexes byElectrospray Ionization Mass Spectrometry and Ion Mobility Analysis(Joseph A. Loo and Catherine S. Kaddis).
1.1 Introduction.
1.2 Critical Aspects of the Experimental Procedure.
1.3 Solution Phase Equilibria and Gas Phase Dissociation.
1.4 Conclusions.
Chapter 2. Softly, Softly-Detection of Protein Complexes byMatrix-Assisted Laser Desorption Ionisation Mass Spectrometry(Kevin M. Downard).
2.1 Introduction.
2.2 First Glimpses and the First-Shot Phenomenon.
2.3 Matrix and Solution Criteria to Preserve ProteinComplexes.
2.4 Laser Fluence, Wavelength and Ion Extraction.
2.5 Preservation of Protein Complexes on Conventional MaldiTargets.
2.6 Affinity Targets and Surfaces Coupled To Maldi.
2.7 Conclusions.
Chapter 3. Probing Protein Interactions UsingHydrogen-Deuterium Exchange Mass Spectrometry (David D. Weis, SumaKaveti, Yan Wu and John R. Engen).
3.1 Introduction.
3.2 Hydrogen Exchange Background.
3.3 General HX-MS Method.
3.4 Interactions of Proteins.
3.5 Examples.
3.6 Conclusions.
Chapter 4. Limited Proteolysis Mass Spectrometry of ProteinComplexes (Maria Monti and Piero Pucci).
4.1 Introduction.
4.2 Limited Proteolysis Analysis.
4.3 Experimental Design.
4.4 Probing Protein-Protein Interactions.
4.5 Probing Protein-Nucleic Acid Interactions.
4.6 Protein-Ligand Interactions.
4.7 Probing Amyloid Fibril Core.
4.8 Conlusions.
Chapter 5. Chemical Cross-Linking and Mass Spectrometry forInvestigation of Protein-Protein Interactions (AndreaSinz).
Introduction.
5.1 Cross-Linking Strategies.
5.2 Functional Groups of Cross-Linking Reagents.
5.3 Mass Spectrometric Analysis of Cross-Linked Products.
5.4 Identification of Cross-Linked Produc.
5.5 Computer Software for Data Analysis.
5.6 Conclusions and Perspectives.
Chapter 6. Genesis & Application of Radical Probe MassSpectrometry (RP-MS) to Study Protein Interactions (Simin D.Maleknia and Kevin M. Downard).
6.1 Genesis of Radical Probe Mass Spectrometry.
6.2 The Reactive Residue Side Chains.
6.3 Conditions Important to Radical Probe Mass SpectrometryExperiments.
6.4 Generation of Radicals on Millisecond Timescales.
6.5 Applications of RP-MS to Studies of ProteinInteractions.
6.6 Onset of Oxidative Damage and Its Implications for ProteinInteractions.
6.7 Application of Radical Oxidation to Study ProteinAssemblies.
6.8 Modeling Protein Complexes with Data from RP-MSExperiments.
6.9 Conclusions.
Index.