Immunofluorescence Protocol to Label Astrocytes from Diverse Model and Non-Model Mammals.- Retro-Orbital Delivery of AAVs for CNS-Wide Astrocyte Targeting.- An Empirical-Theoretical Approach to Determine Astroglial Potassium upon Ischemic Stress.- Quantification of Astrocytic Sodium Signals Using Fluorescence Lifetime Imaging (FLIM).- pH Sensor-Based Engulfment Assays to Measure Phagocytic Activity of Astrocytes.- An Astrocyte Transplantation Method to Investigate Astrocyte Development and Diversity in the CNS.- Primary CNS Cultures with Mixed Neural Cell Types to Study Correlative Effects of High Glucocorticoids on Astrocytes, Oligodendrocytes, and Myelination Markers.- Postnatal Development of Perivascular Astrocytic Processes and Detection of Local mRNA and Translation.- Imaging Techniques for the Analysis of the Blood-Brain Barrier and Gliovascular Unit Dysfunction.- Assessment of Blood-Brain Barrier Integrity Using Dyes and Tracers.- Investigating the Pathology-Related Astroglial Plasticity in the Human Cerebral Cortex.- Super-Resolution Analysis of Astrocyte Morphology Using Expansion Microscopy.- Astrocyte Morphological Adaptations in a Mouse Model of Social Anxiety Disorder.- An In Vitro Assay to Measure Astrocyte-Dependent Synaptic Phagocytosis in Health and Major Depressive Disorder.- Cortical Organotypic Brain Slice Cultures to Examine Sex- and Age-Dependent Astrocyte-Mediated Synaptic Phagocytosis.- Development and Validation of Stress Paradigms to Study Astrocytes in Rodent Models of Stress Disorders.- Cellular Investigation of Astrocytic Neuroendocrine Responses to Stress.- Morphological Investigation of Astrocytic Responses to Stress.- Functional Evaluation of Astrocytic Responses to Stress.- Unveiling Ketamine's Influence on Astrocytic Kir4.1 Channels through Multi-Modal Analysis: Confocal Microscopy, Immunocytochemistry, Fluorescence Analysis, and Electrophysiology.- Revealing and Characterizing Astrocytic Atrophy in an Animal Model of Depression.
