Rolling Circle Amplification (RCA)

Preface.- Part 1. Improved DNA polymerases and new DNA and DNA/RNA ligases useful in RCA.- 1. Improvement of f29 DNA polymerase amplification performance by fusion of DNA binding motifs.- 2. Preparation of circular templates by T4 RNA ligase 2 for rolling circle amplification of target microRNAs with high specificity and sensitivity.- 3. Use of DNA CircLigase for direct isothermal detection of microbial mRNAs by RNA-primed rolling circle amplification and preparation of ø29 DNA polymerase not contaminated.- Part 2. RCA-involving techniques with enhanced signal amplification.- 4. Nicking enzyme-assisted branched-chain RCA reaction for cascade DNA amplification.- 5. Combining isothermal amplification techniques: Coupled RCA-LAMP.- Part 3. Emerging RCA diagnostics.- 6. Detection of vascular disease-related single nucleotide polymorphisms in clinical samples using ramified rolling circle amplification.- 7. Ultrasensitive isothermal detection of protein analytes using rolling circle amplification in micro-scale platforms.- 8. Rolling circle amplification with padlock probes for in situ detection of RNA analytes.- 9. PNA-assisted rolling circle amplification for detection of DNA marker sequences in human cells.- 10. Sensor systems with magnetic and optomagnetic readout of rolling circle amplification products.- Part 4. Prospective RCA-based therapeutics.- 11. DNA nanoclews for stimuli-responsive anticancer drug delivery.- 12. RCA-assisted self-assembled DNA origami nano-constructs as vehicles for cellular delivery of diagnostic probes and therapeutic drugs.- 13. DeNAno: a novel multivalent affinity reagent produced by selection of RCA-generated DNA nanoparticle libraries.- Index.