Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

- Fluorogenic detection of protein aggregates in live cells using the AggTag method Kwan Ho Jung and Xin Zhang - Synthesis and application of a ratiometric probe for hydrogen peroxide Ruwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. Stains - Protein labeling for FRET with methoxycoumarin and acridonylalanine Chloe M. Jones, Yarra Venkatesh and E. James Petersson - Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assays Paul T. Ludford III and Yitzhak Tor - Method to discriminate amyloids using fluorescent probes Kristine L. Teppang, Rachel S. Ehrlich and Jerry Yang - Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives Hui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. Farkas - Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancer John C. Widen, Martina Tholen, Joshua J. Yim and Matthew Bogyo - Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles Nanxi Wang and Lei Wang - 4-Cyanoindole-based fluorophores for biological spectroscopy and microscopy Arusha Acharyya, Ismail A. Ahmed and Feng Gai - Dissecting contributions of catalytic and reader domains in regulation of histone demethylation Nektaria Petronikolou, James E. Longbotham and Danica Galonic Fujimori - Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization Qing Zhang, Yue Zhang and Xuechen Li - Chemical synthesis of di-ubiquitin modified histones for further biochemical studies Jia-Bin Li, Jun Liang and Changlin Tian - Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies Swathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela Chiosis - FlICk (fluorescent isoindole crosslinking) for peptide stapling Mihajlo Todorovic and David M. Perrin - Selective protein N-terminal labeling with N-hydroxysuccinimide esters Hanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. Dempsey - Covalent live-cell labeling of proteins using a photoreactive fluorogen Tewoderos M. Ayele, Steve D. Knutson and Jennifer M. Heemstra - Photoconvertible diazaxanthilidene dyes for live cell imaging Rachel M. Lackner, Christopher L. Johnny and David M. Chenoweth - No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag Wei Sheng, James H. Geiger and Babak Borhan