Presently, the need for methods involving separation, identification, and characterization of different kinds of cells is amply realized among immu nologists, hematologists, cell biologists, clinical pathologists, and cancer researchers. Unless cells exhibiting different functions and stages of differ entiation are separated from one another, it will be exceedingly difficult to study some of the molecular mechanisms involved in cell recognition, specialization, interactions, cytotoxicity, and transformation. Clinical diag nosis of diseased states and use of isolated cells for therapeutic (e. g. , immunotherapy) or survival (e. g. , transfusion) purposes are some of the pressing areas where immediate practical benefits can be obtained by applying cell separation techniques. However. the development of such useful methods is still in its infancy. A number of good techniques exist based either on the physical or biological properties of the cells, and these have produced some valuable results. Still others are to be discovered. Therefore, the purpose of this open-end treatise is to acquaint the reader with some of the basic principles, instrumentation, and procedures pres ently in practice at various laboratories around the world and to present some typical applications of each technique to particular biological prob lems. To this end, I was fortunate to obtain the contribution of certain leading scientists in the field of cell separation, people who in their pioneer ing work have struggled with the particular problems involved in separating living cells and in some way have won.
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Sprache
Verlagsort
Verlagsgruppe
Dateigröße
ISBN-13
978-1-4684-0820-1 (9781468408201)
DOI
10.1007/978-1-4684-0820-1
Schweitzer Klassifikation
1 Preparative Density Gradient Electrophoresis and Velocity Sedimentation at Unit Gravity of Mammalian Cells.- I. Density Gradient Electrophoresis.- II. Velocity Sedimentation at Unit Gravity.- III. Conclusions.- References.- 2 Isopycnic Separation of Cells and Cell Organelles by Centrifugation in Modified Colloidal Silica Gradients.- I. Introduction.- II. Preparations of Colloidal Silica.- III. Methodology.- IV. Centrifugation of Cells.- V. Centrifugation of Subcellular Particles.- VI. Concluding Remarks.- References.- 3 Dielectrophoresis: Applications to the Characterization and Separation of Cells.- I. Introduction.- II. Mechanism of Nonuniform Field Effects and Dielectrophoresis.- III. Polarization Mechanisms in Biological Materials.- IV. Experimental Collection and Separation of Cells.- References.- 4 Separation of Viable Cells by Velocity Sedimentation in an Isokinetic Gradient of Ficoll in Tissue Culture Medium.- I. Introduction.- II. Historical Development of Technique.- III. Isopycnic Sedimentation.- IV. Velocity (Including Isokinetic) Sedimentation in Ficoll Gradients.- V. Selected Theoretical Considerations.- VI. Critical Analysis of Data from Experiments in Cell Separation.- References.- 5 Fractionation and Manipulation of Cells with Chemically Modified Fibers and Surfaces.- I. Introduction.- II. Affinity Fractionation of Cells.- III. Fiber Fractionation of Cells.- IV. Manipulation of Cells and the Study of Localized Perturbations at the Cell Surface.- V. Procedures.- VI. Applications.- VII. Prospective Applications.- References.- 6 The Separation of Lymphoid Cells on the Basis of Physical Parameters: Separation of B- and T-Cell Subsets and Characterization of B-Cell Differentiation Stages.- I. Introduction.- II. Separation of B from T Lymphocytes.- III. Separation of Functionally Distinct B-Lymphocyte Subsets.- IV. General Conclusions.- V. Appendix: Technical Aspects of the Separation Procedures.- References.- 7 Multiparameter Analysis and Sorting of Mammalian Cells.- I. Introduction.- II. Description of the LASL Multiparameter Cell Separator.- III. Examples of Multiparameter Cell Analysis and Sorting Applications.- IV. Summary and Conclusions.- References.- 8 Partition of Cells in Two-Polymer Aqueous Phases: A Surface Affinity Method for Cell Separation.- I. Introduction.- II. Methods.- III. Results.- IV. Conclusion.- References.
