This volume provides a complete and timely guide to the use of adeno-associated virus (AAV) vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory systems, and to a broad range of other tissues. Novel techniques such as ultrasound-targeted delivery to the brain, subpial delivery to the spinal cord, and subILM delivery to the retina are accompanied by chapters that provide an overview and comparison of current methods for AAV delivery to tissues such as brain, heart, liver, and lung. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive,
Adeno-Associated Virus Vectors: Design and Delivery
aims to enhance the utility of AAV vectors for targeted gene transfer to living animals and continue the ongoing development of novel AAV-based gene therapies for human disease.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
50
12 s/w Abbildungen, 50 farbige Abbildungen
XII, 426 p. 62 illus., 50 illus. in color.
Dateigröße
ISBN-13
978-1-4939-9139-6 (9781493991396)
DOI
10.1007/978-1-4939-9139-6
Schweitzer Klassifikation
1. Design of AAV vectors for Delivery of RNAi.- 2. Design of AAV Vectors for Delivery of Large or Multiple Transgenes.- 3. Ligand Coupling to the AAV Capsid for Cell-Specific Gene Transfer.- 4. Quantitative and Digital Droplet-based AAV Genome Titration.- 5. Single-Stranded DNA Virus Sequencing (SSV-Seq) for Characterization of Residual.- 6. In Situ Hybridization for Detection of AAV-Mediated Gene Expression.- 7. Use of AAV Vectors for CRISPR-Mediated In Vivo Genome Editing in the Retina.- 8. Intravenous Infusion of AAV for Widespread Gene Delivery to the Nervous System.- 9. Intraspinal and Intracortical Delivery of AAV Vectors for Intersectional Circuit Tracing in Non-Transgenic Species.- 10. MRI-Guided Focused Ultrasound for Targeted Delivery of rAAV to the Brain.- 11. AAV-Mediated Gene Delivery to the Spinal Cord by Intrathecal Injection.- 12. Subpial AAV Delivery for Spinal Parenchymal Gene Regulation in Adult Mammals.- 13. Peripheral AAV Injection for Retrograde Transduction of Dorsal Root and
Trigeminal Ganglia.- 14. SubILM Injection of AAV for Gene Delivery to the Retina.- 15. Intracameral Delivery of AAV to Corneal Endothelium for Expression of Secretory Proteins.- 16. AAV-Mediated Gene Delivery to the Inner Ear.- 17. Intranasal Delivery of Adenoviral and AAV Vectors for Transduction of the Mammalian Peripheral Olfactory System.- 18. AAV-Mediated Gene Delivery to Taste Cells of the Tongue.- 19. AAV Vectors for Efficient Gene Delivery to Rodent Hearts.- 20. AAV-Mediated Gene Delivery to the Liver: Overview of Current Technologies and Methods.- 21.- AAV-Mediated Gene Delivery to the Lung.- 22. Simplified Purification of AAV and Delivery to the Pancreas by Intraductal Administration.- 23. rAAV-Mediated Gene Delivery to Adipose Tissue.- 24. AAV-Mediated Gene Delivery to the Enteric Nervous System by Intracolonic Injection.
