Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology

1. Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches2. Enzymatic isomerization of ¿-carotene mediated by the heme-containing isomerase Z-ISO3. Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes4. Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids5. Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages6. Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis7. The isolation of sub-chromoplast structures from tomato and capsicum fruit8. Carrot protoplasts as a suitable method for protein subcellular localization9. High throughput production and characterization of carotenoid enzymes for structural and functional studies10. Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation11. Characterizing CYTOCHROME P450 Enzymes Involved in Plant Apocarotenoid Metabolism by Using Engineered Yeast System12. Preparation of carotenoid cleavage dioxygenases for crystallography13. Heme-dependent enzymes involved in carotenoids biosynthesis in plants14. Tracking sub-plastidic localization of carotenoid metabolic enzymes with proteomics15. Golden Rice and lessons learned for inspiring future metabolic engineering strategies and synthetic biology solutions16. Genetic transformation of Cassava to improve carotenoids: a protocol revisited17. Elevating fruit carotenoid content in apple (Malus x domestica Borkh)18. Engineering microbial synthesis of carotenoids19. The breeder's tool-box for enhancing the content of esterified carotenoids in wheat: from extraction and profiling of carotenoids to marker-assisted selection of candidate genes20. Drawing on enzyme promiscuity to create novel carotenoids via non-phytoene precursors21. Plant abscisic acid receptor agonists and antagonists22. A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi23. Designing biosensors from a plant apocarotenoid receptor24. Imaging Retinaldehyde-Protein Binding in Plants using a Merocyanine Reporter 25. Use of directed enzyme evolution to create novel biosynthetic pathways for production of non-natural and rare carotenoids