Mechanisms of Gene Regulation: How Science Works

Central dogma of molecular biology.- Impact of chromatin structure.- Epigenetics enables gene expression.- Gene regulation in the context of nuclear architecture.- Core promoter.- TATA box and other core promoter elements.- Genome-wide core promoter identification.- TFIID and Mediator as paradigms of multi-protein complexes.- Site-specific transcription factors and their domains.- Classification of transcription factors.- Activation of transcription factors.- Programing cellular differentiation by transcription factors.- Inflammatory signaling via NF-KB.- Sensing cellular stress via p53.- The nuclear receptor superfamily.- Molecular interactions of nuclear receptors.- Physiological role of nuclear receptors.- Next-generation sequencing.- Gene regulation in the context of Big Biology.- Exploring genome-wide transcription factor binding.- Integrating epigenome-wide datasets.- Cytosines and their methylation.- Histone modifications.- Gene regulation via chromatin modifiers.- Sensing energy metabolism via chromatin modifiers.- Epigenetics and chromatin.- Genome-wide understanding of epigenetics.- CTCF and genetic imprinting.- Epigenetics in health and disease.- Nucleosome positioning at promoters.- Chromatin remodeling.- Transcriptional dynamics in the presence of chromatin.- Organization of the nucleus.- Non-coding RNAs.- miRNAs and their regulatory potential.- Long ncRNAs.- Enhancer RNAs.