Non-Ribosomal Peptide Biosynthesis and Engineering

The Assembly-line Enzymology of Nonribosomal Peptide Biosynthesis.- Structural Studies of Modular Nonribosomal Peptide Synthetases.- Using NMR Titration to Study Protein-Protein Interactions in AcpP Dependent FAS-II.- Chemoproteomic Profiling of Adenylation Domain Functions in Gramicidin S-producing Nonribosomal Peptide Synthetases.- In Vitro Biochemical Characterization of Excised Macrocyclizing Thioesterase Domains from Non-Ribosomal Peptide Synthetases.- Chemo-Enzymatic Synthesis of Non-Ribosomal Macrolactams by a Penicillin-binding Protein-type Thioesterase.- Directed Evolution of the BpsA Carrier Protein Domain for Recognition by Non-cognate 4'-Phosphopantetheinyl Transferases to Enable Inhibitor Screening.- Unraveling Structural Information of Multi-Domain Nonribosomal Peptide Synthetases by Using Photo-Crosslinking Analysis with Genetic Code Expansion.- A Chemoenzymatic Approach to Investigate Cytochrome P450 Crosslinking in Glycopeptide Antibiotic Biosynthesis.- Cross-Linking of the Nonribosomal Peptide Synthetase Adenylation Domain with a Carrier Protein Using a Pantetheine-Type Probe.- A Practical Guideline to Engineering Non-Ribosomal Peptide Synthetases.- Probing Substrate-Loaded Carrier Proteins by Nuclear Magnetic Resonance.- Ribosomal Synthesis of Peptides Bearing Noncanonical Backbone Structures via Chemical posttranslational modifications.- Thioester Capture Strategy for the Identification of Nonribosomal Peptide and Polyketide Intermediates.- Chemical Labeling of Protein 4´-Phosphopantetheinylation in Surfactin-Producing Nonribosomal Peptide Synthetases.- Norine: Bioinformatics Methods and Tools for the Characterization of Newly Discovered NonRibosomal Peptides.