Synthetic Biology

Plant engineering to enable platforms for sustainable bioproduction of terpenoids.- Compartmentalized Terpenoid Production in Plants Using Agrobacterium-mediated Transient Expression.- Design Principles for Biological Adaptation: A Systems and Control-Theoretic Treatment.- Construction of Xylose-Utilizing Cyanobacterial Chassis for Bioproduction Under Photomixotrophic Conditions.- Allosteric-Regulation-Based DNA Circuits In Saccharomyces Cerevisiae To Detect Organic Acids And Monitor Hydrocarbon Metabolism In Vitro.- dCas12a:Pre-crRNA: A New Tool To Induce mRNA Degradation In Saccharomyces Cerevisiae Synthetic Gene Circuits.- CRISPRi-Driven Genetic Screening for Designing Novel Microbial Phenotypes.- Enzymatic Preparation of DNA with an Expanded Genetic Alphabet Using Terminal Deoxynucleotidyl Transferase and Its Applications.- Single-Nucleotide Microbial Genome Editing Using CRISPR-Cas12a.- Multiplex Marker-less Genome Integration in Pichiapastoris using CRISPR/Cas9.- Genome Editing, Transcriptional Regulation and Forward Genetic Screening Using CRISPR-Cas12a Systems in Yarrowia lipolytica.- An Improved Method for Eliminating or Creating Intragenic Bacterial Promoters.- Genetic Code Expansion in Pseudomonas putida KT2440.- Genome-Wide Screen for Enhanced Noncanonical Amino Acid Incorporation in Yeast.- Positive Selection Screens for Programmable Endonuclease Activity using I-SceI.- CRISPR-Cas9-mediated Genome Editing in Paenibacillus polymyxa.- Programming Juxtacrine-Based Synthetic Signaling Networks in a Cellular Potts Framework.- Encoding Genetic Circuits with DNA Barcodes Paves the Way for High-throughput Profiling of Dose-response Curves of Metabolite Biosensors.- Machine Learning for Biological Design.- A machine learning approach for predicting essentiality of metabolic genes.- The Causes for Genomic Instability And How To Try And Reduce Them Through Rational Design Of Synthetic DNA.- Genetic Network Design Automation with LOICA.- Flapjack: Data Management and Analysis for Genetic Circuit Characterization.- In Vivo DNA Assembly Using the PEDA Method.- Cell-Free Synthesis and Quantitation of Bacteriophages.- Multimodal Control of Bacterial Gene Expression by Red and Blue Light.- In silico design, in vitro construction and in vivo application of synthetic small regulatory RNAs in bacteria.