This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded glycoproteins and those found in other pathogens, the book continues by examining the production of mammalian glycoproteins, the analysis of glycoprotein content, cell-free synthesis of glycoproteins and considerations for production and purification of glycoproteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Recombinant Glycoproteins: Methods and Protocols provides a wide range of guidelines for studying these vitally important proteins.
Chapter "Solubilization of Oligomeric Cell-Free Synthesized Proteins Using SMA Copolymers" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
12
77 farbige Abbildungen, 12 s/w Abbildungen
XIV, 355 p. 89 illus., 77 illus. in color.
Dateigröße
ISBN-13
978-1-0716-3666-4 (9781071636664)
DOI
10.1007/978-1-0716-3666-4
Schweitzer Klassifikation
